The translation inhibitor anisomycin induces Elk-1-mediated transcriptional activation of egr-1 through multiple mitogen-activated protein kinase pathways
DC Field | Value | Language |
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dc.contributor.author | Shin, Soon Young | - |
dc.contributor.author | Lee, Joon Ho | - |
dc.contributor.author | Min, Byung Wook | - |
dc.contributor.author | Lee, Young Han | - |
dc.date.available | 2020-11-03T13:44:56Z | - |
dc.date.issued | 2006-12-31 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18456 | - |
dc.description.abstract | The early growth response-1 gene (egr-1) encodes a zinc-finger transcription factor Egr-1 and is rapidly inducible by a variety of extracellular stimuli. Anisomycin (ANX), a protein synthesis inhibitor, stimulates mitogen-activated protein kinase (MAPK) pathways and thereby causes a rapid induction of immediate-early response genes. We found that anisomycin treatment of U87MG glioma cells resulted in a marked, time-dependent increase in levels of Egr-1 protein. The results of Northern blot analysis and reporter gene assay of egr-1 gene promoter (Pegr-1) activity indicate that the ANX-induced increase in Egr-1 occurs at the transcriptional level. Deletion of the serum response element (SRE) in the 5'-flanking region of egr-1 gene abolished ANX-induced Pegr-1 activity. ANX induced the phosphorylation of the ERK1/2, JNK, and p38 MAPKs in a time-dependent manner and also induced transactivation of Gal4-Elk-1, suggesting that Elk-1 is involved in SRE-mediated egr-1 transcription. Transient transfection of dominant-negative constructs of MAPK pathways blocked ANX-induced P,g,l activity. Furthermore, pretreatment with specific MAPK pathway inhibitors, including the MEK inhibitor U0126, the JNK inhibitor SP600125, and the p38 kinase inhibitor SB2021190, completely inhibited ANX-inducible expression of Egr-1. Taken together, these results suggest that all three MAPK pathways play a crucial role in ANX-induced transcriptional activation Of Pegr-1 through SRE-mediated transactivation of Elk-1. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | The translation inhibitor anisomycin induces Elk-1-mediated transcriptional activation of egr-1 through multiple mitogen-activated protein kinase pathways | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1038/emm.2006.80 | - |
dc.identifier.scopusid | 2-s2.0-33846236901 | - |
dc.identifier.wosid | 000243345000011 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, v.38, no.6, pp 677 - 685 | - |
dc.citation.title | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.citation.volume | 38 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 677 | - |
dc.citation.endPage | 685 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001185620 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | TERNARY COMPLEX FACTORS | - |
dc.subject.keywordPlus | C-FOS GENE | - |
dc.subject.keywordPlus | SERUM RESPONSE ELEMENTS | - |
dc.subject.keywordPlus | IMMEDIATE-EARLY GENE | - |
dc.subject.keywordPlus | GROWTH-FACTOR | - |
dc.subject.keywordPlus | DIFFERENTIAL REGULATION | - |
dc.subject.keywordPlus | FACTORS ELK-1 | - |
dc.subject.keywordPlus | HT1080 CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | anisomycin | - |
dc.subject.keywordAuthor | cycloheximide | - |
dc.subject.keywordAuthor | early growth response protein 1 | - |
dc.subject.keywordAuthor | ets-domain protein Elk-1 | - |
dc.subject.keywordAuthor | mitogen-activated protein kinases | - |
dc.subject.keywordAuthor | serum response element | - |
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