Induction of immunity against Hepatitis B virus surface antigen by intranasal DNA vaccination using a cationic emulsion as a mucosal gene carrier
DC Field | Value | Language |
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dc.contributor.author | Kim, Tae Woo | - |
dc.contributor.author | Chung, Hesson | - |
dc.contributor.author | Kwon, Ick Chan | - |
dc.contributor.author | Sung, Ha Chin | - |
dc.contributor.author | Kang, Tae Heung | - |
dc.contributor.author | Han, Hee Dong | - |
dc.contributor.author | Jeong, Seo Young | - |
dc.date.available | 2020-11-03T13:46:41Z | - |
dc.date.issued | 2006-10-31 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.issn | 0219-1032 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18567 | - |
dc.description.abstract | Delivery of DNA vaccines to airway mucosa would be an ideal method for mucosal immunization. However, there have been few reports of a suitable gene delivery system. In this study we used a cationic emulsion to immunize mice via the intranasal route with pCMV-S coding for Hepatitis B virus surface antigen (HBsAg). Complexing pCMV-S with a cationic emulsion dramatically enhanced HBsAg expression in both nasal tissue and lung, and was associated with increases in the levels of HBs-specific Abs in serum and mucosal fluids, of cytotoxic T lymphocytes (CTL) in the spleen and cervical and iliac lymph nodes, and of delayed-type hypersensitivity (DTH) against HBsAg. In contrast, very weak humoral and cellular immunities were observed following immunization with naked DNA. In support of these observations, a higher proliferative response of spleenocytes was detected in the group immunized with the emulsion/pCMV-S complex than in the group immunized with naked pCMV-S. These findings may facilitate development of an emulsion-mediated gene vaccination technique for use against intracellular pathogens that invade mucosal surfaces. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER SINGAPORE PTE LTD | - |
dc.title | Induction of immunity against Hepatitis B virus surface antigen by intranasal DNA vaccination using a cationic emulsion as a mucosal gene carrier | - |
dc.type | Article | - |
dc.publisher.location | 싱가폴 | - |
dc.identifier.scopusid | 2-s2.0-33847650537 | - |
dc.identifier.wosid | 000241798100008 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.22, no.2, pp 175 - 181 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 22 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 175 | - |
dc.citation.endPage | 181 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | VACCINES | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | SARS | - |
dc.subject.keywordAuthor | cationic lipid emulsion | - |
dc.subject.keywordAuthor | Hepatitis B virus | - |
dc.subject.keywordAuthor | mucosal DNA vaccine | - |
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