Phospholipase C-γ1 is a guanine nucleotide exchange factor for dynamin-1 and enhances dynamin-1-dependent epidermal growth factor receptor endocytosis
DC Field | Value | Language |
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dc.contributor.author | Choi J.H. | - |
dc.contributor.author | Park J.B. | - |
dc.contributor.author | Bae S.S. | - |
dc.contributor.author | Yun S. | - |
dc.contributor.author | Kim H.S. | - |
dc.contributor.author | Hong W.-P. | - |
dc.contributor.author | Kim I.-S. | - |
dc.contributor.author | Kim J.H. | - |
dc.contributor.author | Han M.Y. | - |
dc.contributor.author | Ryu S.H. | - |
dc.contributor.author | Patterson R.L. | - |
dc.contributor.author | Synder S.H. | - |
dc.contributor.author | Suh P.-G. | - |
dc.date.available | 2020-11-03T17:51:47Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.issn | 1477-9137 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/20868 | - |
dc.description.abstract | Phospholipase C-γ1 (PLC-γ1), which interacts with a variety of signaling molecules through its two Src homology (SH) 2 domains and a single SH3 domain has been implicated in the regulation of many cellular functions. We demonstrate that PLC-γ1 acts as a guanine nucleotide exchange factor (GEF) of dynamin-1, a 100 kDa GTPase protein, which is involved in clathrin-mediated endocytosis of epidermal growth factor (EGF) receptor. Overexpression of PLC-γ1 increases endocytosis of the EGF receptor by increasing guanine nucleotide exchange activity of dynamin-1. The GEF activity of PLC-γ1 is mediated by the direct interaction of its SH3 domain with dynamin-1. EGF-dependent activation of ERK and serum response element (SRE) are both up-regulated in PC12 cells stably overexpressing PLC-γ1, but knockdown of PLC-γ1 by ARNA significantly reduces ERK activation. These results establish a new role for PLC-γ1 in the regulation of endocytosis and suggest that endocytosis of activated EGF receptors may mediate PLC-γ 1-dependent proliferation. | - |
dc.format.extent | 11 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Phospholipase C-γ1 is a guanine nucleotide exchange factor for dynamin-1 and enhances dynamin-1-dependent epidermal growth factor receptor endocytosis | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1242/jcs.01220 | - |
dc.identifier.scopusid | 2-s2.0-4444234130 | - |
dc.identifier.bibliographicCitation | Journal of Cell Science, v.117, no.17, pp 3785 - 3795 | - |
dc.citation.title | Journal of Cell Science | - |
dc.citation.volume | 117 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 3785 | - |
dc.citation.endPage | 3795 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | clathrin | - |
dc.subject.keywordPlus | dynamin I | - |
dc.subject.keywordPlus | epidermal growth factor receptor | - |
dc.subject.keywordPlus | guanine nucleotide exchange factor | - |
dc.subject.keywordPlus | mitogen activated protein kinase | - |
dc.subject.keywordPlus | phospholipase C gamma1 | - |
dc.subject.keywordPlus | small interfering RNA | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | dynamin I | - |
dc.subject.keywordPlus | epidermal growth factor receptor | - |
dc.subject.keywordPlus | guanine nucleotide exchange factor | - |
dc.subject.keywordPlus | guanosine triphosphate | - |
dc.subject.keywordPlus | phospholipase C | - |
dc.subject.keywordPlus | phospholipase C gamma | - |
dc.subject.keywordPlus | small interfering RNA | - |
dc.subject.keywordPlus | animal cell | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell proliferation | - |
dc.subject.keywordPlus | cell strain | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | endocytosis | - |
dc.subject.keywordPlus | enzyme activation | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | protein domain | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein function | - |
dc.subject.keywordPlus | protein protein interaction | - |
dc.subject.keywordPlus | rat | - |
dc.subject.keywordPlus | regulatory mechanism | - |
dc.subject.keywordPlus | serum responsive element | - |
dc.subject.keywordPlus | upregulation | - |
dc.subject.keywordPlus | animal | - |
dc.subject.keywordPlus | brain | - |
dc.subject.keywordPlus | dose response | - |
dc.subject.keywordPlus | endocytosis | - |
dc.subject.keywordPlus | genetic transcription | - |
dc.subject.keywordPlus | immunoprecipitation | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | physiology | - |
dc.subject.keywordPlus | protein tertiary structure | - |
dc.subject.keywordPlus | reporter gene | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | Src homology domain | - |
dc.subject.keywordPlus | time | - |
dc.subject.keywordPlus | Animalia | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Brain | - |
dc.subject.keywordPlus | Cell Proliferation | - |
dc.subject.keywordPlus | Clathrin | - |
dc.subject.keywordPlus | Dose-Response Relationship, Drug | - |
dc.subject.keywordPlus | Dynamin I | - |
dc.subject.keywordPlus | Endocytosis | - |
dc.subject.keywordPlus | Genes, Reporter | - |
dc.subject.keywordPlus | Guanine Nucleotide Exchange Factors | - |
dc.subject.keywordPlus | Guanosine Triphosphate | - |
dc.subject.keywordPlus | Immunoprecipitation | - |
dc.subject.keywordPlus | PC12 Cells | - |
dc.subject.keywordPlus | Phospholipase C | - |
dc.subject.keywordPlus | Phospholipase C gamma | - |
dc.subject.keywordPlus | Protein Structure, Tertiary | - |
dc.subject.keywordPlus | Rats | - |
dc.subject.keywordPlus | Receptor, Epidermal Growth Factor | - |
dc.subject.keywordPlus | RNA, Small Interfering | - |
dc.subject.keywordPlus | Signal Transduction | - |
dc.subject.keywordPlus | src Homology Domains | - |
dc.subject.keywordPlus | Time Factors | - |
dc.subject.keywordPlus | Transcription, Genetic | - |
dc.subject.keywordPlus | Up-Regulation | - |
dc.subject.keywordAuthor | Dynamin-1 | - |
dc.subject.keywordAuthor | Endocytosis | - |
dc.subject.keywordAuthor | Guanine nucleotide exchange factor (GEF) | - |
dc.subject.keywordAuthor | Phospholipase C-γ1 | - |
dc.subject.keywordAuthor | Proliferation | - |
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