Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seo, Ha-Rim | - |
dc.contributor.author | Joo, Hyung Joon | - |
dc.contributor.author | Kim, Dae Hwan | - |
dc.contributor.author | Cui, Long-Hui | - |
dc.contributor.author | Choi, Seung-Cheol | - |
dc.contributor.author | Kim, Jong-Ho | - |
dc.contributor.author | Cho, Sung Woo | - |
dc.contributor.author | Lee, Kyu Back | - |
dc.contributor.author | Lim, Do-Sun | - |
dc.date.available | 2020-11-02T08:41:51Z | - |
dc.date.issued | 2017-05-24 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.issn | 1944-8252 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/4982 | - |
dc.description.abstract | Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1(+) MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1(+). MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres, With mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized enhanced cardiomyocyte differentiation in Flk1(+) MPCs. | - |
dc.format.extent | 10 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/acsami.7b01555 | - |
dc.identifier.scopusid | 2-s2.0-85019685957 | - |
dc.identifier.wosid | 000402498600009 | - |
dc.identifier.bibliographicCitation | ACS APPLIED MATERIALS & INTERFACES, v.9, no.20, pp 16804 - 16813 | - |
dc.citation.title | ACS APPLIED MATERIALS & INTERFACES | - |
dc.citation.volume | 9 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 16804 | - |
dc.citation.endPage | 16813 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | EMBRYONIC STEM-CELLS | - |
dc.subject.keywordPlus | CARDIOVASCULAR PROGENITORS | - |
dc.subject.keywordPlus | NANOTOPOGRAPHY | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | TOPOGRAPHY | - |
dc.subject.keywordPlus | MORPHOLOGY | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | Flk1-positive mesodermal precursor cells | - |
dc.subject.keywordAuthor | gradient nanopattern plates | - |
dc.subject.keywordAuthor | cardiomyocyte differentiation | - |
dc.subject.keywordAuthor | cytoskeleton reorganization | - |
dc.subject.keywordAuthor | nanoimprintiug | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
73, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea (02841)82-2-2286-1265
COPYRIGHT 2020 KOREA UNIVERSITY MEDICAL LIBRARY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.