Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) assay for on-site diagnosis of SARS CoV-2
DC Field | Value | Language |
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dc.contributor.author | Jang, Woong Sik | - |
dc.contributor.author | Lim, Da Hye | - |
dc.contributor.author | Yoon, Jung | - |
dc.contributor.author | Kim, Ahran | - |
dc.contributor.author | Lim, Minsup | - |
dc.contributor.author | Nam, Jeonghun | - |
dc.contributor.author | Yanagihara, Richard | - |
dc.contributor.author | Ryu, Sook-Won | - |
dc.contributor.author | Jung, Bo Kyeung | - |
dc.contributor.author | Ryoo, Nam-Hee | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.date.accessioned | 2021-05-17T01:41:11Z | - |
dc.date.available | 2021-05-17T01:41:11Z | - |
dc.date.issued | 2021-03-03 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/52601 | - |
dc.description.abstract | A newly identified coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), has spread rapidly from its epicenter in China to more than 150 countries across six continents. In this study, we have designed three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) primer sets to detect the RNA-dependent RNA polymerase (RdRP), Envelope (E) and Nucleocapsid protein (N) genes of SARS CoV-2. For one tube reaction, the detection limits for five combination SARS CoV-2 LAMP primer sets (RdRP/E, RdRP/N, E/N, RdRP/E/N and RdRP/N/Internal control (actin beta)) were evaluated with a clinical nasopharyngeal swab sample. Among the five combination, the RdRP/E and RdRP/N/IC multiplex LAMP assays showed low detection limits. The sensitivity and specificity of the RT-LAMP assay were evaluated and compared to that of the widely used Allplex (TM) 2019-nCoV Assay (Seegene, Inc., Seoul, South Korea) and PowerChek (TM) 2019-nCoV Real-time PCR kit (Kogenebiotech, Seoul, South Korea) for 130 clinical samples from 91 SARS CoV-2 patients and 162 NP specimens from individuals with (72) and without (90) viral respiratory infections. The multiplex RdRP (FAM)/N (CY5)/IC (Hex) RT-LAMP assay showed comparable sensitivities (RdRP: 93.85%, N: 94.62% and RdRP/N: 96.92%) to that of the Allplex (TM) 2019-nCoV Assay (100%) and superior to those of PowerChek (TM) 2019-nCoV Real-time PCR kit (RdRP: 92.31%, E: 93.85% and RdRP/E: 95.38%). | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) assay for on-site diagnosis of SARS CoV-2 | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1371/journal.pone.0248042 | - |
dc.identifier.scopusid | 2-s2.0-85102453975 | - |
dc.identifier.wosid | 000625981500040 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.16, no.3 | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 16 | - |
dc.citation.number | 3 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
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