Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by the gamma-Ray Irradiation Technique
DC Field | Value | Language |
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dc.contributor.author | Yun, Young-Pil | - |
dc.contributor.author | Lee, Jae Yong | - |
dc.contributor.author | Jeong, Won Jae | - |
dc.contributor.author | Park, Kyeongsoon | - |
dc.contributor.author | Kim, Hak-Jun | - |
dc.contributor.author | Song, Jae-Jun | - |
dc.contributor.author | Kim, Sung Eun | - |
dc.contributor.author | Song, Hae-Ryong | - |
dc.date.available | 2020-11-02T16:44:18Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 2314-6133 | - |
dc.identifier.issn | 2314-6141 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/8593 | - |
dc.description.abstract | The purpose of this study was to demonstrate the ability of BMP-2-immobilized polycaprolactone (PCL) fibers modified using the gamma-ray irradiation technique to induce the osteogenic differentiation of MG-63 cells. Poly acrylic acid (AAc) was grafted onto the PCL fibers by the gamma-ray irradiation technique. BMP-2 was then subsequently immobilized onto the AAc-PCL fibers (BMP2/AAc-PCL). PCL and surface-modified PCL fibers was characterized by evaluation with a scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and contact angle. The biological activity of the PCL and surface-modified PCL fibers were characterized by alkaline phosphatase (ALP) activity, calcium deposition, and them RNA expression of osteocalcin and osteopontin in MG-63 cells. Successfully grafted AAc and PCL fibers with immobilized BMP-2 were confirmed by XPS results. The results of the contact angle showed that BMP-2/AAc-PCL fibers have more hydrophilic properties in comparison to PCL fibers. The ALP activity, calcium deposition, and gene expressions of MG-63 cells grown on BMP-2/AAc-PCL fibers showed greatly induced osteogenic differentiation in comparison to the PCL fibers. In conclusion, these results demonstrated that BMP-2/AAc-PCL fibers have the potential to effectively induce the osteogenic differentiation of MG-63 cells. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | HINDAWI LTD | - |
dc.title | Improving Osteogenesis Activity on BMP-2-Immobilized PCL Fibers Modified by the gamma-Ray Irradiation Technique | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1155/2015/302820 | - |
dc.identifier.scopusid | 2-s2.0-84930654989 | - |
dc.identifier.wosid | 000355435700001 | - |
dc.identifier.bibliographicCitation | BIOMED RESEARCH INTERNATIONAL, v.2015 | - |
dc.citation.title | BIOMED RESEARCH INTERNATIONAL | - |
dc.citation.volume | 2015 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | BONE MORPHOGENETIC PROTEIN-2 | - |
dc.subject.keywordPlus | MARROW STROMAL CELLS | - |
dc.subject.keywordPlus | PEPTIDE-AMPHIPHILE NANOFIBERS | - |
dc.subject.keywordPlus | SURFACE MODIFICATION | - |
dc.subject.keywordPlus | OSTEOBLASTIC DIFFERENTIATION | - |
dc.subject.keywordPlus | PLASMA TREATMENT | - |
dc.subject.keywordPlus | BMP-2 | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
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