The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus
DC Field | Value | Language |
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dc.contributor.author | Kim, Jin Il | - |
dc.contributor.author | Hwang, Min-Woong | - |
dc.contributor.author | Lee, Ilseob | - |
dc.contributor.author | Park, Sehee | - |
dc.contributor.author | Lee, Sangmoo | - |
dc.contributor.author | Bae, Joon-Yong | - |
dc.contributor.author | Heo, Jun | - |
dc.contributor.author | Kim, Donghwan | - |
dc.contributor.author | Jang, Seok-Il | - |
dc.contributor.author | Park, Mee Sook | - |
dc.contributor.author | Kwon, Hyung-Joo | - |
dc.contributor.author | Song, Jin-Won | - |
dc.contributor.author | Park, Man-Seong | - |
dc.date.available | 2020-11-02T17:46:38Z | - |
dc.date.issued | 2014-06 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/9199 | - |
dc.description.abstract | By nature of their segmented RNA genome, influenza A viruses (IAVs) have the potential to generate variants through a reassortment process. The influenza nonstructural (NS) gene is critical for a virus to counteract the antiviral responses of the host. Therefore, a newly acquired NS segment potentially determines the replication efficiency of the reassortant virus in a range of different hosts. In addition, the C-terminal PDZ-binding motif (PBM) has been suggested as a pathogenic determinant of IAVs. To gauge the pandemic potential from human and avian IAV reassortment, we assessed the replication properties of NS-reassorted viruses in cultured cells and in the lungs of mice and determined their transmissibility in guinea pigs. Compared with the recombinant A/Korea/01/2009 virus (rK09; 2009 pandemic H1N1 strain), the rK09/VN:NS virus, in which the NS gene was adopted from the A/Vietnam/1203/2004 virus (a human isolate of the highly pathogenic avian influenza H5N1 virus strains), exhibited attenuated virulence and reduced transmissibility. However, the rK09/VN:NS-PBM virus, harboring the PBM in the C-terminus of the NS1 protein, recovered the attenuated virulence of the rK09/VN:NS virus. In a guinea pig model, the rK09/VN:NS-PBM virus showed even greater transmission efficiency than the rK/09 virus. These results suggest that the PBM in the NS1 protein may determine viral persistence in the human and avian IAV interface. (C) 2014 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Academic Press | - |
dc.title | The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.bbrc.2014.04.132 | - |
dc.identifier.scopusid | 2-s2.0-84901818136 | - |
dc.identifier.wosid | 000337990700004 | - |
dc.identifier.bibliographicCitation | Biochemical and Biophysical Research Communications, v.449, no.1, pp 19 - 25 | - |
dc.citation.title | Biochemical and Biophysical Research Communications | - |
dc.citation.volume | 449 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 19 | - |
dc.citation.endPage | 25 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.subject.keywordPlus | A VIRUS | - |
dc.subject.keywordPlus | KIDNEY-CELLS | - |
dc.subject.keywordPlus | H1N1 VIRUS | - |
dc.subject.keywordPlus | INTERFERON | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | VIRULENCE | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DELETION | - |
dc.subject.keywordAuthor | Influenza A virus | - |
dc.subject.keywordAuthor | Interferon | - |
dc.subject.keywordAuthor | NS1 protein | - |
dc.subject.keywordAuthor | PDZ-binding motif | - |
dc.subject.keywordAuthor | Transmission | - |
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