Cyclic Stretching Induces Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes through Nuclear-Mechanotransduction
DC Field | Value | Language |
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dc.contributor.author | Song, Myeongjin | - |
dc.contributor.author | Jang, Yongjun | - |
dc.contributor.author | Kim, Seung-Jong | - |
dc.contributor.author | Park, Yongdoo | - |
dc.date.accessioned | 2022-03-21T02:40:14Z | - |
dc.date.available | 2022-03-21T02:40:14Z | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 1738-2696 | - |
dc.identifier.issn | 2212-5469 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/55450 | - |
dc.description.abstract | Background: During cardiogenesis, cardiac cells receive various stimuli, such as biomechanical and chemical cues, from the surrounding microenvironment, and these signals induce the maturation of heart cells. Mechanical force, especially tensile force in the heart, is one of the key stimuli that induce cardiomyocyte (CM) maturation through mechanotransduction, a process through which physical cues are transformed into biological responses. However, the effects and mechanisms of tensile force on cell maturation are poorly studied. Methods: In this study, we developed a cyclic stretch system that mimics the mechanical environment of the heart by loading tensile force to human-induced pluripotent stem cell (hiPSC)-derived CMs. hiPSC-CMs cultured with the cyclic stretch system analyzed morphological change, immunofluorescent staining, expression of maturation markers in mRNA, and beating properties compared to static cultures. Results: hiPSC-CMs cultured with the cyclic stretch system showed increased cell alignment, sarcomere length and expression of maturation markers in mRNA, such as TNNI3, MYL2 and TTN, compared to static cultures. Especially, the expression of genes related to nuclear mechanotransduction, such as Yap1, Lamin A/C, plectin, and desmin, was increased in the cyclically stretched hiPSC-CMs. Furthermore, the volume of the nucleus was increased by as much as 120% in the cyclic stretch group. Conclusion: These results revealed that nuclear mechanotransduction induced by tensile force is involved in CM maturation. Together, these findings provide novel evidence suggesting that nuclear mechanotransduction induced by tensile force is involved in the regulation of cardiac maturation. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국조직공학과 재생의학회 | - |
dc.title | Cyclic Stretching Induces Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes through Nuclear-Mechanotransduction | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1007/s13770-021-00427-z | - |
dc.identifier.scopusid | 2-s2.0-85125941536 | - |
dc.identifier.wosid | 000766059300001 | - |
dc.identifier.bibliographicCitation | Tissue Engineering and Regenerative Medicine, v.19, no.4, pp 781 - 792 | - |
dc.citation.title | Tissue Engineering and Regenerative Medicine | - |
dc.citation.volume | 19 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 781 | - |
dc.citation.endPage | 792 | - |
dc.type.docType | Article; Early Access | - |
dc.identifier.kciid | ART002866500 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.subject.keywordPlus | CARDIAC-HYPERTROPHY | - |
dc.subject.keywordPlus | FORCE | - |
dc.subject.keywordPlus | TISSUES | - |
dc.subject.keywordPlus | MATRIX | - |
dc.subject.keywordAuthor | Tensile force | - |
dc.subject.keywordAuthor | Cyclic stretch | - |
dc.subject.keywordAuthor | Cardiomyocyte | - |
dc.subject.keywordAuthor | Maturation | - |
dc.subject.keywordAuthor | Nuclear-mechanotransduction | - |
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