Differential dependency of human glioblastoma cells on vascular endothelial growth factor-A signaling via neuropilin-1
DC Field | Value | Language |
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dc.contributor.author | Lee, Jungwhoi | - |
dc.contributor.author | Chong, Kyuha | - |
dc.contributor.author | Lee, Jungsul | - |
dc.contributor.author | Kim, Chungyeul | - |
dc.contributor.author | Kim, Jae-Hoon | - |
dc.contributor.author | Choi, Kyungsun | - |
dc.contributor.author | Choi, Chulhee | - |
dc.date.accessioned | 2022-10-05T00:40:13Z | - |
dc.date.available | 2022-10-05T00:40:13Z | - |
dc.date.issued | 2022-10 | - |
dc.identifier.issn | 1019-6439 | - |
dc.identifier.issn | 1791-2423 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61529 | - |
dc.description.abstract | Despite the high expression of neuropilin-1 (NRP-1) in human glioblastoma (GB), the understanding of its function as a co-receptor of vascular endothelial growth factor receptors (VEGFRs) in angiogenesis is currently limited. Therefore, the aim of the present study was to elucidate the non-classical function of NRP-1 expression in human GB. Expression patterns of NRP-1 and VEGF-A were determined by sandwich ELISA, western blot analysis, or immunohistochemistry. Differential dependency of GB cells following ablation of VEGF-A signaling was validated in vitro and in vivo. Cellular mechanism responsible for distinct response to VEGF-A signaling was evaluated by western blotting and immune-precipitation analysis. Prognostic implications were assessed using IHC analysis. GB cells exhibited differing sensitivity to silencing of vascular endothelial growth factor (VEGF)-A signaling, which resulted in a distinct expression pattern of wild-type or chondroitin-sulfated NRP-1. VEGF-A-sensitive GB exhibited the physical interaction between wild-type NRP-1 and FMS related receptor tyrosine kinase 1 (Flt-1) whereas VEGF-A-resistant GB exhibited chondroitin-sulfated NRP-1 without interaction with Flt-1. Eliminating the chondroitin sulfate modification in NRP-1 led to re-sensitization to VEGF-A signaling, and chondroitin sulfate modification was found to be associated with an adverse prognosis in patients with GB. The present study identified the distinct functions of NRP-1 in VEGF-A signaling in accordance with its unique expression type and interaction with Flt-1. The present research is expected to provide a strong basis for targeting VEGF-A signaling in patients with GB, with variable responses. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Demetrios A. Spandidos Ed. & Pub. | - |
dc.title | Differential dependency of human glioblastoma cells on vascular endothelial growth factor-A signaling via neuropilin-1 | - |
dc.type | Article | - |
dc.publisher.location | 그리이스 | - |
dc.identifier.doi | 10.3892/ijo.2022.5412 | - |
dc.identifier.scopusid | 2-s2.0-85136967796 | - |
dc.identifier.wosid | 000857096600001 | - |
dc.identifier.bibliographicCitation | International Journal of Oncology, v.61, no.4 | - |
dc.citation.title | International Journal of Oncology | - |
dc.citation.volume | 61 | - |
dc.citation.number | 4 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | CHONDROITIN SULFATE | - |
dc.subject.keywordPlus | FACTOR VEGF | - |
dc.subject.keywordPlus | AUTOCRINE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordAuthor | neuropilin-1 | - |
dc.subject.keywordAuthor | glioblastoma | - |
dc.subject.keywordAuthor | chondroitin sulfate | - |
dc.subject.keywordAuthor | FMS related receptor tyrosine kinase | - |
dc.subject.keywordAuthor | autocrine signaling | - |
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