Exposure to airborne particulate matter induces renal tubular cell injury in vitro: The role of vitamin D signaling and renin-angiotensin system
DC Field | Value | Language |
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dc.contributor.author | Kang, Eungu | - |
dc.contributor.author | Yim, Hyung Eun | - |
dc.contributor.author | Nam, Yoon Jeong | - |
dc.contributor.author | Jeong, Sang Hoon | - |
dc.contributor.author | Kim, Joo-Ae | - |
dc.contributor.author | Lee, Ju-Han | - |
dc.contributor.author | Son, Min Hwa | - |
dc.contributor.author | Yoo, Kee Hwan | - |
dc.date.accessioned | 2022-10-31T02:40:44Z | - |
dc.date.available | 2022-10-31T02:40:44Z | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 2405-8440 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61689 | - |
dc.description.abstract | Background Exposure to air pollution can interfere with the vitamin D endocrine system. This study investigated the effects of airborne particulate matter (PM) on renal tubular cell injury in vitro and explored the underlying mechanisms. Methods HK-2 human renal proximal tubule cells were treated with PM with or without 1,25(OH)2D3 analog, 19-Nor-1,25(OH)2D2 (paricalcitol, 10 nM) for 48 h. The dose- and time-dependent cytotoxicity of PM with or without paricalcitol was determined via cell counting kit-8 assay. Cellular oxidative stress was assessed using commercially available enzyme-linked immunosorbent assay kits. The protein expression of vitamin D receptor (VDR), cytochrome P450(CYP)27B1, CYP24A1, renin, angiotensin converting enzyme (ACE), angiotensin II type 1 receptor (AT1), nuclear factor erythroid 2-related factor 2 (Nrf2), nuclear factor-kB (NF-kB), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 was determined. Results PM exposure decreased HK-2 cell viability in a dose- and time-dependent manner. The activities of superoxide dismutase and malondialdehyde in HK-2 cells increased significantly in the group exposed to PM. PM exposure decreased VDR and Nrf2, while increasing CYP27B1, renin, ACE, AT1, NF-kB, TNF-α, and IL-6. The expression of VDR, CYP27B1, renin, ACE, AT1, and TNF-α was reversed by paricalcitol treatment. Paricalcitol also restored the cell viability of PM-exposed HK-2 cells. Conclusion Our findings indicate that exposure to PM induces renal proximal tubular cell injury, concomitant with alteration of vitamin D endocrine system and renin angiotensin system. Vitamin D could attenuate renal tubular cell damage following PM exposure by suppressing the renin-angiotensin system and by partially inhibiting the inflammatory response. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Elsevier BV | - |
dc.title | Exposure to airborne particulate matter induces renal tubular cell injury in vitro: The role of vitamin D signaling and renin-angiotensin system | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1016/j.heliyon.2022.e10184 | - |
dc.identifier.scopusid | 2-s2.0-85135966934 | - |
dc.identifier.wosid | 000865378400009 | - |
dc.identifier.bibliographicCitation | Heliyon, v.8, no.8 | - |
dc.citation.title | Heliyon | - |
dc.citation.volume | 8 | - |
dc.citation.number | 8 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | D-RECEPTOR | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | AIR-POLLUTION | - |
dc.subject.keywordPlus | D METABOLISM | - |
dc.subject.keywordPlus | KIDNEY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | Airborne Particulate Matter | - |
dc.subject.keywordAuthor | Kidney Tubules | - |
dc.subject.keywordAuthor | Renin-Angiotensin System | - |
dc.subject.keywordAuthor | Vitamin D Deficiency | - |
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