Engineered Human Intervertebral Disc Model Inducing Degenerative Microglial Proinflammation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hwang, Min-Ho | - |
dc.contributor.author | Kang, You Jung | - |
dc.contributor.author | Son, Hyeong-Guk | - |
dc.contributor.author | Cho, Hansang | - |
dc.contributor.author | Choi, Hyuk | - |
dc.date.accessioned | 2022-11-14T02:40:35Z | - |
dc.date.available | 2022-11-14T02:40:35Z | - |
dc.date.issued | 2022-10 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.issn | 1422-0067 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61768 | - |
dc.description.abstract | Degeneration of the intervertebral disc (IVD) is a major contributor to low back pain (LBP). IVD degeneration is characterized by abnormal production of inflammatory cytokines secreted by IVD cells. Although the underlying molecular mechanisms of LBP have not been elucidated, increasing evidence suggests that LBP is associated particularly with microglia in IVD tissues and the peridiscal space, aggravating the cascade of degenerative events. In this study, we implemented our microfluidic chemotaxis platform to investigate microglial inflammation in response to our reconstituted degenerative IVD models. The IVD models were constructed by stimulating human nucleus pulposus (NP) cells with interleukin-1 beta and producing interleukin-6 (129.93 folds), interleukin-8 (18.31 folds), C-C motif chemokine ligand-2 (CCL-2) (6.12 folds), and CCL-5 (5.68 folds). We measured microglial chemotaxis (p < 0.05) toward the conditioned media of the IVD models. In addition, we observed considerable activation of neurodegenerative and deactivation of protective microglia via upregulated expression of CD11b (p < 0.001) and down-regulation of CD206 protein (p < 0.001) by soluble factors from IVD models. This, in turn, enhances the inflammatory milieu in IVD tissues, causing matrix degradation and cellular damage. Our findings indicate that degenerative IVD may induce degenerative microglial proinflammation, leading to LBP development. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Engineered Human Intervertebral Disc Model Inducing Degenerative Microglial Proinflammation | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/ijms232012216 | - |
dc.identifier.scopusid | 2-s2.0-85140815493 | - |
dc.identifier.wosid | 000872943900001 | - |
dc.identifier.bibliographicCitation | International Journal of Molecular Sciences, v.23, no.20 | - |
dc.citation.title | International Journal of Molecular Sciences | - |
dc.citation.volume | 23 | - |
dc.citation.number | 20 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | RAT MODEL | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PAINFUL | - |
dc.subject.keywordPlus | HERNIATION | - |
dc.subject.keywordPlus | IL-1-BETA | - |
dc.subject.keywordPlus | PROFILE | - |
dc.subject.keywordAuthor | intervertebral disc degeneration | - |
dc.subject.keywordAuthor | human nucleus pulposus | - |
dc.subject.keywordAuthor | microglia | - |
dc.subject.keywordAuthor | proinflammation | - |
dc.subject.keywordAuthor | chemotaxis | - |
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