Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition
DC Field | Value | Language |
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dc.contributor.author | Sim, Ju-Ri | - |
dc.contributor.author | Shin, Dong Hoon | - |
dc.contributor.author | Park, Pil-Gu | - |
dc.contributor.author | Park, So-Hyeon | - |
dc.contributor.author | Bae, Joon-Yong | - |
dc.contributor.author | Lee, Youngchae | - |
dc.contributor.author | Kang, Dha-Yei | - |
dc.contributor.author | Kim, Ye Jin | - |
dc.contributor.author | Aum, Sowon | - |
dc.contributor.author | Noh, Shin Hye | - |
dc.contributor.author | Hwang, Su Jin | - |
dc.contributor.author | Cha, Hye-Ran | - |
dc.contributor.author | Kim, Cheong Bi | - |
dc.contributor.author | Ko, Si Hwan | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Jeon, Dongkyu | - |
dc.contributor.author | Cho, Sungwoo | - |
dc.contributor.author | Lee, Gee Eun | - |
dc.contributor.author | Kim, Jeonghun | - |
dc.contributor.author | Moon, Young-hye | - |
dc.contributor.author | Kim, Jae-Ouk | - |
dc.contributor.author | Nam, Jae-Sung | - |
dc.contributor.author | Kim, Chang-Hoon | - |
dc.contributor.author | Moon, Sungmin | - |
dc.contributor.author | Chung, Youn Wook | - |
dc.contributor.author | Park, Man-Seong | - |
dc.contributor.author | Ryu, Ji-Hwan | - |
dc.contributor.author | Namkung, Wan | - |
dc.contributor.author | Lee, Jae Myun | - |
dc.contributor.author | Lee, Min Goo | - |
dc.date.accessioned | 2022-12-05T01:40:46Z | - |
dc.date.available | 2022-12-05T01:40:46Z | - |
dc.date.issued | 2022-07 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.issn | 2211-1247 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61913 | - |
dc.description.abstract | As an enveloped virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) delivers its viral genome into host cells via fusion of the viral and cell membranes. Here, we show that ANO6/TMEM16F-mediated cell surface exposure of phosphatidylserine is critical for SARS-CoV-2 entry and that ANO6-selective inhibitors are effective against SARS-CoV-2 infections. Application of the SARS-CoV-2 Spike pseudotyped virus (SARS2-PsV) evokes a cytosolic Ca2+ elevation and ANO6-dependent phosphatidylserine externalization in ACE2/TMPRSS2-positive mammalian cells. A high-throughput screening of drug-like chemical libraries iden-tifies three different structural classes of chemicals showing ANO6 inhibitory effects. Among them, A6-001 dis-plays the highest potency and ANO6 selectivity and it inhibits the single-round infection of SARS2-PsV in ACE2/ TMPRSS2-positive HEK 293T cells. More importantly, A6-001 strongly inhibits authentic SARS-CoV-2-induced phosphatidylserine scrambling and SARS-CoV-2 viral replications in Vero, Calu-3, and primarily cultured human nasal epithelial cells. These results provide mechanistic insights into the viral entry process and offer a potential target for pharmacological intervention to protect against coronavirus disease 2019 (COVID-19). | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Cell Press | - |
dc.title | Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.celrep.2022.111117 | - |
dc.identifier.scopusid | 2-s2.0-85134741257 | - |
dc.identifier.wosid | 000883795600007 | - |
dc.identifier.bibliographicCitation | Cell Reports, v.40, no.3 | - |
dc.citation.title | Cell Reports | - |
dc.citation.volume | 40 | - |
dc.citation.number | 3 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | CORONAVIRUS SPIKE PROTEIN | - |
dc.subject.keywordPlus | ENTRY DEPENDS | - |
dc.subject.keywordPlus | SARS-COV | - |
dc.subject.keywordPlus | FUSION | - |
dc.subject.keywordPlus | CHANNEL | - |
dc.subject.keywordPlus | EXPOSURE | - |
dc.subject.keywordPlus | FORMS | - |
dc.subject.keywordAuthor | ANO6/TMEM16F | - |
dc.subject.keywordAuthor | CP: Microbiology | - |
dc.subject.keywordAuthor | phosphatidylserine | - |
dc.subject.keywordAuthor | SARS-CoV-2 | - |
dc.subject.keywordAuthor | virus-cell fusion | - |
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