Rapid Detection of Mycobacterium Tuberculosis Using a Novel Point-of-Care BZ TB/NTM NALF Assay: Integrating LAMP and LFIA Technologies
DC Field | Value | Language |
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dc.contributor.author | Kim, Ha Nui | - |
dc.contributor.author | Lee, Junmin | - |
dc.contributor.author | Yoon, Soo-Young | - |
dc.contributor.author | Jang, Woong Sik | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.date.accessioned | 2023-06-12T02:40:10Z | - |
dc.date.available | 2023-06-12T02:40:10Z | - |
dc.date.issued | 2023-04 | - |
dc.identifier.issn | 2075-4418 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/63243 | - |
dc.description.abstract | Tuberculosis (TB) is one of the leading causes of infectious mortality from a single infectious agent, Mycobacterium tuberculosis (MTB). This study evaluated the performance of the newly developed BZ TB/NTM NALF assay, which integrated loop-mediated isothermal amplification and lateral flow immunochromatographic assay technologies, for the detection of MTB. A total of 80 MTB-positive samples and 115 MTB-negative samples were collected, all of which were confirmed by TB real-time PCR (RT-PCR) using either AdvanSure (TM) TB/NTM RT-PCR Kit or Xpert((R)) MTB/RIF Assay. The performance of the BZ TB/NTM NALF assay was evaluated by calculating its sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) in comparison to those of the RT-PCR methods. Compared to the RT-PCR, the sensitivity, specificity, PPV, and NPV of BZ TB/NTM NALF assay were 98.7%, 99.1%, 98.7%, and 99.1%, respectively. The concordance rate between BZ TB/NTM NALF and RT-PCR was 99.0%. Rapid and simple detection of MTB is essential for global case detection and further elimination of TB. The performance of the BZ TB/NTM NALF Assay is acceptable with a high concordance with RT-PCR, indicating that it is reliable for use in a low-resource environment. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | MDPI AG | - |
dc.title | Rapid Detection of Mycobacterium Tuberculosis Using a Novel Point-of-Care BZ TB/NTM NALF Assay: Integrating LAMP and LFIA Technologies | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/diagnostics13081497 | - |
dc.identifier.scopusid | 2-s2.0-85153926481 | - |
dc.identifier.wosid | 000979529300001 | - |
dc.identifier.bibliographicCitation | Diagnostics, v.13, no.8 | - |
dc.citation.title | Diagnostics | - |
dc.citation.volume | 13 | - |
dc.citation.number | 8 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | LABORATORY DIAGNOSIS | - |
dc.subject.keywordPlus | SEQUENCE | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | BIAS | - |
dc.subject.keywordAuthor | tuberculosis | - |
dc.subject.keywordAuthor | TB | - |
dc.subject.keywordAuthor | MTB | - |
dc.subject.keywordAuthor | NALF | - |
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