Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyun-Ah | - |
dc.contributor.author | Kim, Hyunsoo | - |
dc.contributor.author | Nam, Min-Kyung | - |
dc.contributor.author | Park, Jong Kook | - |
dc.contributor.author | Lee, Moo-Yeal | - |
dc.contributor.author | Chung, Seok | - |
dc.contributor.author | Lee, Kyung-Mi | - |
dc.contributor.author | Kuh, Hyo-Jeong | - |
dc.date.accessioned | 2023-10-16T08:40:12Z | - |
dc.date.available | 2023-10-16T08:40:12Z | - |
dc.date.issued | 2023-09 | - |
dc.identifier.issn | 1475-2867 | - |
dc.identifier.issn | 1475-2867 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/64163 | - |
dc.description.abstract | BackgroundRecently, natural killer (NK) cells emerged as a treatment option for various solid tumors. However, the immunosuppressive tumor immune microenvironment (TIME) can reduce the cytotoxic ability of NK cells in pancreatic ductal adenocarcinoma. Cancer-associated fibroblasts within the tumor stroma can suppress immune surveillance by dysregulating factors involved in the cellular activity of NK cells. Herein, the effect of activated pancreatic stellate cells (aPSCs) on NK cell-mediated anticancer efficacy under three-dimensional (3D) coculture conditions was investigated.Methods3D cocultures of PANC-1 tumor spheroids (TSs) with aPSCs and NK-92 cells in a collagen matrix were optimized to identify the occurring cellular interactions and differential cytokine profiles in conditioned media using microchannel chips. PANC-1 TSs and aPSCs were indirectly cocultured, whereas NK-92 cells were allowed to infiltrate the TS channel using convective medium flow.ResultsCoculture with aPSCs promoted PANC-1 TSs growth and suppressed the antitumor cytotoxic effects of NK-92 cells. Mutual inhibition of cellular activity without compromising migration ability was observed between aPSCs and NK-92 cells. Moreover, the reduced killing activity of NK-92 cells was found to be related with reduced granzyme B expression in NK cells.ConclusionsHerein, a novel TIME-on-chip model based on the coculture of PANC-1 TSs, aPSCs, and NK-92 cells was described. This model may be useful for studying the detailed mechanisms underlying NK cells dysregulation and for exploring future therapeutic interventions to restore NK cell activity in the tumor microenvironment. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central | - |
dc.title | Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/s12935-023-03064-9 | - |
dc.identifier.scopusid | 2-s2.0-85172448942 | - |
dc.identifier.wosid | 001075083400002 | - |
dc.identifier.bibliographicCitation | Cancer Cell International, v.23, no.1 | - |
dc.citation.title | Cancer Cell International | - |
dc.citation.volume | 23 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | TUMOR MICROENVIRONMENT | - |
dc.subject.keywordPlus | IL-6 | - |
dc.subject.keywordAuthor | Natural killer cell | - |
dc.subject.keywordAuthor | Pancreatic stellate cell | - |
dc.subject.keywordAuthor | Pancreatic ductal adenocarcinoma | - |
dc.subject.keywordAuthor | Tumor spheroid | - |
dc.subject.keywordAuthor | Tumor immune microenvironment | - |
dc.subject.keywordAuthor | 3D coculture system | - |
dc.subject.keywordAuthor | TIME-on-chip model | - |
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