Targeted deletion of CD244 on monocytes promotes differentiation into anti-tumorigenic macrophages and potentiates PD-L1 blockade in melanoma
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jeongsoo | - |
dc.contributor.author | Kim, Tae-Jin | - |
dc.contributor.author | Chae, Sehyun | - |
dc.contributor.author | Ha, Hyojeong | - |
dc.contributor.author | Park, Yejin | - |
dc.contributor.author | Park, Sunghee | - |
dc.contributor.author | Yoon, Chul Joo | - |
dc.contributor.author | Lim, Seon Ah | - |
dc.contributor.author | Lee, Hyemin | - |
dc.contributor.author | Kim, Ji Young | - |
dc.contributor.author | Kim, Jungwon | - |
dc.contributor.author | Im, Kyungtaek | - |
dc.contributor.author | Lee, Kyunghye | - |
dc.contributor.author | Kim, Jeongmin | - |
dc.contributor.author | Kim, Daham | - |
dc.contributor.author | Lee, Eunju | - |
dc.contributor.author | Shin, Min Hwa | - |
dc.contributor.author | Park, Serk In | - |
dc.contributor.author | Rhee, Inmoo | - |
dc.contributor.author | Jung, Keehoon | - |
dc.contributor.author | Lee, Jeewon | - |
dc.contributor.author | Lee, Keun Hwa | - |
dc.contributor.author | Hwang, Daehee | - |
dc.contributor.author | Lee, Kyung-Mi | - |
dc.date.accessioned | 2024-03-27T02:30:06Z | - |
dc.date.available | 2024-03-27T02:30:06Z | - |
dc.date.issued | 2024-02 | - |
dc.identifier.issn | 1476-4598 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/65810 | - |
dc.description.abstract | BackgroundIn the myeloid compartment of the tumor microenvironment, CD244 signaling has been implicated in immunosuppressive phenotype of monocytes. However, the precise molecular mechanism and contribution of CD244 to tumor immunity in monocytes/macrophages remains elusive due to the co-existing lymphoid cells expressing CD244.MethodsTo directly assess the role of CD244 in tumor-associated macrophages, monocyte-lineage-specific CD244-deficient mice were generated using cre-lox recombination and challenged with B16F10 melanoma. The phenotype and function of tumor-infiltrating macrophages along with antigen-specific CD8 T cells were analyzed by flow cytometry and single cell RNA sequencing data analysis, and the molecular mechanism underlying anti-tumorigenic macrophage differentiation, antigen presentation, phagocytosis was investigated ex vivo. Finally, the clinical feasibility of CD244-negative monocytes as a therapeutic modality in melanoma was confirmed by adoptive transfer experiments.ResultsCD244fl/flLysMcre mice demonstrated a significant reduction in tumor volume (61% relative to that of the CD244fl/fl control group) 14 days after tumor implantation. Within tumor mass, CD244fl/flLysMcre mice also showed higher percentages of Ly6Clow macrophages, along with elevated gp100+IFN-gamma+ CD8 T cells. Flow cytometry and RNA sequencing data demonstrated that ER stress resulted in increased CD244 expression on monocytes. This, in turn, impeded the generation of anti-tumorigenic Ly6Clow macrophages, phagocytosis and MHC-I antigen presentation by suppressing autophagy pathways. Combining anti-PD-L1 antibody with CD244-/- bone marrow-derived macrophages markedly improved tumor rejection compared to the anti-PD-L1 antibody alone or in combination with wild-type macrophages. Consistent with the murine data, transcriptome analysis of human melanoma tissue single-cell RNA-sequencing dataset revealed close association between CD244 and the inhibition of macrophage maturation and function. Furthermore, the presence of CD244-negative monocytes/macrophages significantly increased patient survival in primary and metastatic tumors.ConclusionOur study highlights the novel role of CD244 on monocytes/macrophages in restraining anti-tumorigenic macrophage generation and tumor antigen-specific T cell response in melanoma. Importantly, our findings suggest that CD244-deficient macrophages could potentially be used as a therapeutic agent in combination with immune checkpoint inhibitors. Furthermore, CD244 expression in monocyte-lineage cells serve as a prognostic marker in cancer patients. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central | - |
dc.title | Targeted deletion of CD244 on monocytes promotes differentiation into anti-tumorigenic macrophages and potentiates PD-L1 blockade in melanoma | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/s12943-024-01936-w | - |
dc.identifier.scopusid | 2-s2.0-85186284483 | - |
dc.identifier.wosid | 001178592800001 | - |
dc.identifier.bibliographicCitation | Molecular Cancer, v.23, no.1 | - |
dc.citation.title | Molecular Cancer | - |
dc.citation.volume | 23 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | SLAM FAMILY RECEPTORS | - |
dc.subject.keywordPlus | T-CELLS | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | SPECIFICITY | - |
dc.subject.keywordPlus | ACQUISITION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | INHIBITORS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SUBSETS | - |
dc.subject.keywordAuthor | CD244 | - |
dc.subject.keywordAuthor | Macrophages | - |
dc.subject.keywordAuthor | Differentiation | - |
dc.subject.keywordAuthor | Macrophage-based cell therapy | - |
dc.subject.keywordAuthor | Melanoma | - |
dc.subject.keywordAuthor | Immune checkpoint blockade | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
73, Goryeodae-ro, Seongbuk-gu, Seoul, Republic of Korea (02841)82-2-2286-1265
COPYRIGHT 2020 KOREA UNIVERSITY MEDICAL LIBRARY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.