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Cited 17 time in webofscience Cited 16 time in scopus
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Expression of Heat Shock Protein 70 Modulates the Chemoresponsiveness of Pancreatic Cancer

Authors
Hyun, Jong JinLee, Hong SikKeum, BoraSeo, Yeon SeokJeen, Yoon TaeChun, Hoon JaiUm, Soon HoKim, Chang Duck
Issue Date
Nov-2013
Publisher
EDITORIAL OFFICE GUT & LIVER
Keywords
Gemcitabine; Heat shock proteins; Pancreatic neoplasms; Quercetin
Citation
GUT AND LIVER, v.7, no.6, pp.739 - 746
Indexed
SCIE
SCOPUS
KCI
OTHER
Journal Title
GUT AND LIVER
Volume
7
Number
6
Start Page
739
End Page
746
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/10211
DOI
10.5009/gnl.2013.7.6.739
ISSN
1976-2283
Abstract
Background/Aims: Heat shock protein (HSP) 70 is constitutively overexpressed in pancreatic cancer cells (PCCs) and appears to confer protection against chemotherapeutics. We investigated whether modulating HSP 70 increases chemoresponsiveness to gemcitabine in PCCs. Methods: Varying concentrations of quercetin and gemcitabine, either alone or in combination, were added to PCCs (Panc-1 and MiaPaCa-2). MU assay was performed to analyze cell viability. HSP 70 expression was assessed by Western blot analysis. Apoptosis was determined by measuring caspase-3 activity. Western blot for the LC3-II protein detected the presence of autophagy. Results: HSP 70 levels were not affected by the incubation of Panc-1 and MiaPaCa-2 cells with gemcitabine, whereas with quercetin, the levels were reduced in both cell lines. The viability of both Panc-1 and MiaPaCa-2 cells significantly decreased with gemcitabine treatment but not with quercetin. A combination of gemcitabine and quercetin decreased the viability of both cell lines in a dose-dependent manner, which was more pronounced than gemcitabine treatment alone. Treatment with either gemcitabine or quercetin augmented caspase-3 activity in both cell lines, and a combination of these compounds further potentiated caspase-3 activity. LC3-II protein expression was negligible with gemcitabine treatment but marked with quercetin. The addition of gemcitabine to quercetin did not Potentiate LC3-II protein expression. Conclusions: Modulation of HSP 70 expression with quercetin enhanced the chemoresponsiveness of PCCs to gemcitabine. The mechanism of cell death was both apoptosis and autophagy.
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2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles

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Lee, Hong Sik
안암병원 (Department of Gastroenterology and Hepatology, Anam Hospital)
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