Effects of alginate hydrogels on In Vitro maturation outcome of mouse preantral follicles
- Authors
- Park K.E.; Kim Y.Y.; Ku S.-Y.; Baek S.M.; Huh Y.; Kim Y.J.; Kim S.H.; Choi Y.M.; Moon S.Y.
- Issue Date
- Jun-2012
- Publisher
- KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
- Keywords
- Alginate; Extracellular matrix; Follicle; In vitro maturation; Ovary
- Citation
- Tissue Engineering and Regenerative Medicine, v.9, no.3, pp 170 - 174
- Pages
- 5
- Indexed
- SCIE
SCOPUS
KCICANDI
- Journal Title
- Tissue Engineering and Regenerative Medicine
- Volume
- 9
- Number
- 3
- Start Page
- 170
- End Page
- 174
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/12730
- DOI
- 10.1007/s13770-012-0170-x
- ISSN
- 1738-2696
2212-5469
- Abstract
- Ovarian follicle growth and oocyte maturation require communications between the oocyte and its surrounding somatic cells. Three-dimensional structures provide cell culture conditions that increase interactions between the cellular components when compared to 2-dimensional structures. In this study, alginate matrices were employed to maintain a 3-dimensional architecture to examine its effects on in vitro culture outcomes using mouse ovarian follicles. Ovaries were obtained from the 14-day-old C57BL/6 mice, and preantral follicles were isolated and cultured in either a 2-dimensional culture system or 0.125/0.25% alginate matrices-based 3-dimensional culture systems. We successfully observed encapsulation within 0.125 and 0.25% alginate matrices, which maintained the follicular spherical architecture, with a centrally placed oocyte and layers of granulosa cells. All culture outcomes were comparable between the 2-dimensional control and 3-dimensional alginate group. However, 0.25% alginate hydrogels led the lower rates of follicle survival and antral formation in comparison to the 2-dimensional control and 0.125% alginate. These results demonstrate that a non-permissive stiffness from the high concentration of alginate could interfere with cell-to-cell communication of oocytes and follicles. The extracellular matrix interacts with the oocyte and granulosa cells, resulting in direct effects on their proliferation. The development of a 3-dimensional culture system can enhance follicle maturation and produce meiotically-competent oocytes to provide advanced reproductive options for IVF patients in the future. © Springer, Part of Springer Science+Business Media.
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Collections - 2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles
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