Detailed Information

Cited 7 time in webofscience Cited 7 time in scopus
Metadata Downloads

Is Intercellular Space Different Among Layers in Normal Esophageal Mucosa? An Electron Microscopic Study

Authors
Park, SanghoonChun, Hoon JaiJang, Jin SuKeum, BoraSeo, Yeon SeokKim, Yong SikJeen, Yoon-TaeLee, Hong SikUm, Soon HoKim, Chang DuckRyu, Ho SangUhm, Chang-SubLee, Sung Joon
Issue Date
Dec-2011
Publisher
SPRINGER
Keywords
Esophagus; Gastroesophageal reflux disease; Transmission electron microscopy; Intercellular space; Inter; bserver variation
Citation
DIGESTIVE DISEASES AND SCIENCES, v.56, no.12, pp.3492 - 3497
Indexed
SCIE
SCOPUS
Journal Title
DIGESTIVE DISEASES AND SCIENCES
Volume
56
Number
12
Start Page
3492
End Page
3497
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/12965
DOI
10.1007/s10620-011-1804-x
ISSN
0163-2116
Abstract
Background/Introduction Dilatation of intercellular space (IS) of esophageal epithelial cells is described as a sensitive early marker for epithelial damage by refluxate. Esophageal epithelia are morphologically subdivided into three layers according to the shape of the cells and nuclei. Meanwhile, ten transmission electron microscopy (TEM) photographs and ten randomly selected measurements per photo from gastroesophageal reflux disease (GERD) patients have been widely accepted without any theoretical criticism. We assumed that the IS differs among each layer and thus studied IS according to subdivided layers of normal esophageal epithelium. We also evaluated an optimal number of IS measurements per photograph. Materials and Methods Esophagogastroduodenoscopy was performed in 15 healthy adults without any symptom of GERD, taking biopsies from mucosa above 5 cm from the squamo-columnar junction. Tissues were handled and prepared for TEM, verifying three layers of esophageal mucosa, i.e., squamous cell layer, prickle cell layer, and basal layer. Ten digital photomicrographs were taken from each three layers by TEM, and ISs were measured with a computerized image analysis program. For the method of measuring IS, 5, 10, 20, 30, and 40 measurements per photomicrograph were respectively performed by four different examiners. Mean value and intraclass correlation coefficient (ICC) was also yielded. Results Mean IS of lower esophagus irrelevant to three epithelial layers were 0.39 +/- A 0.30 mu m. When subdivided into three layers, however, mean IS of squamous cell layer was 0.62 +/- A 0.23 mu m, prickle cell layer 0.23 +/- A 0.19 mu m, and basal layer 0.55 +/- A 0.36 mu m, with their difference statistically significant (p < 0.05). On the other hand, ICC of 5, 10, 20, 30, and 40 measurements were 0.688, 0.917, 0.837, 0.790, and 0.765, respectively. Conclusions Mean IS values of each three layers of esophageal epithelium in normal subjects were significantly different, and reconsideration of the standard measurement method is needed. Ten measurements per photo had an adequate inter-observer agreement.
Files in This Item
There are no files associated with this item.
Appears in
Collections
1. Basic Science > Department of Anatomy > 1. Journal Articles
2. Clinical Science > Department of Internal Medicine > 1. Journal Articles
5. Others > Others(Medicine) > 1. Journal Articles
2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Ryu, Ho Sang photo

Ryu, Ho Sang
Anam Hospital (Department of Internal Medicine, Anam Hospital)
Read more

Altmetrics

Total Views & Downloads

BROWSE