Sheathless Shape-Based Separation of Candida Albicans Using a Viscoelastic Non-Newtonian Fluid
- Authors
- Nam, Jeonghun; Jee, Hyunseul; Jang, Woong Sik; Yoon, Jung; Park, Borae G.; Lee, Seong Jae; Lim, Chae Seung
- Issue Date
- Dec-2019
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- sheathless; viscoelastic fluid; separation; shape; Candida
- Citation
- Micromachines, v.10, no.12
- Indexed
- SCIE
SCOPUS
- Journal Title
- Micromachines
- Volume
- 10
- Number
- 12
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/1385
- DOI
- 10.3390/mi10120817
- ISSN
- 2072-666X
- Abstract
- Rapid and accurate identification of Candida albicans from among other candida species is critical for cost-effective treatment and antifungal drug assays. Shape is a critical biomarker indicating cell type, cell cycle, and environmental conditions; however, most microfluidic techniques have been focused only on size-based particle/cell manipulation. This study demonstrates a sheathless shape-based separation of particles/cells using a viscoelastic non-Newtonian fluid. The size of C. albicans was measured at 37 degrees C depending on the incubation time (0 h, 1 h, and 2 h). The effects of flow rates on the flow patterns of candida cells with different shapes were examined. Finally, 2-h-incubated candida cells with germ tube formations (>= 26 mu m) were separated from spherical candida cells and shorter candida cells with a separation efficiency of 80.9% and a purity of 91.2% at 50 mu L/min.
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- Appears in
Collections - 2. Clinical Science > Department of Laboratory Medicine > 1. Journal Articles
- 4. Research institute > Institute for Trauma Research > 1. Journal Articles
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