Endothelin-1 Expression by Vascular Endothelial Growth Factor in Human Umbilical Vein Endothelial Cells and Aortic Smooth Muscle Cells
- Authors
- Seol, Hyun-Joo; Oh, Min-Jeong; Kim, Hai-Joong
- Issue Date
- 2011
- Publisher
- INFORMA HEALTHCARE
- Keywords
- Vascular endothelial growth factor; Endothelin-1; Aortic smooth muscle cells; Human umbilical vein endothelial cells
- Citation
- HYPERTENSION IN PREGNANCY, v.30, no.3, pp 295 - 301
- Pages
- 7
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- HYPERTENSION IN PREGNANCY
- Volume
- 30
- Number
- 3
- Start Page
- 295
- End Page
- 301
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/14198
- DOI
- 10.3109/10641950903115053
- ISSN
- 1064-1955
- Abstract
- Objective. This study investigated endothelin-1 (ET-1) production induced by vascular endothelial growth factor (VEGF) in two different vascular wall cell types. Methods. We analyzed the effect of endothelin-converting enzyme-1 (ECE-1) inhibitor and tissue inhibitors of matrix metalloproteinase-2 (TIMP-2) on VEGF-induced ET-1 expression using real-time polymerase chain reaction and enzyme-linked immunosorbent assay in human umbilical vein endothelial cells and aortic smooth muscle cells. Results. In human umbilical vein endothelial cells, both phosphoramidon, an inhibitor of ECE-1, and TIMP-2 decreased VEGF-induced ET-1 production. In aortic smooth muscle cells, TIMP-2 suppressed VEGF-induced ET-1 production, but phosphoramidon did not influence ET-1 concentration in culture. Conclusion. VEGF-induced ET-1 production may be MMP-2- or ECE-1-dependant in endothelial cells; however, in smooth muscle cells, ET-1 expression appears to be induced by MMP-2 only.
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Collections - 2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles
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