Secretory Profiles and Wound Healing Effects of Human Amniotic Fluid-Derived Mesenchymal Stem Cells
- Authors
- Yoon, Byung Sun; Moon, Jai-Hee; Jun, Eun Kyoung; Kim, Jonggun; Maeng, Isaac; Kim, Jun Sung; Lee, Jung Han; Baik, Cheong Soon; Kim, Aeree; Cho, Kyoung Shik; Lee, Jang Ho; Lee, Hwang Heui; Whang, Kwang Youn; You, Seungkwon
- Issue Date
- Jun-2010
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- STEM CELLS AND DEVELOPMENT, v.19, no.6, pp 887 - 902
- Pages
- 16
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- STEM CELLS AND DEVELOPMENT
- Volume
- 19
- Number
- 6
- Start Page
- 887
- End Page
- 902
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/14799
- DOI
- 10.1089/scd.2009.0138
- ISSN
- 1547-3287
1557-8534
- Abstract
- Recent evidence shows that amniotic fluid (AF) contains multiple cell types derived from the developing fetus, and may represent a novel source of stem cells for cell therapy. In this study, we examined the paracrine factors released by human amniotic fluid-derived mesenchymal stem cells (AF-MSCs) and their ability to accelerate the wound-healing process by stimulating proliferation and migration of dermal fibroblasts. AF-MSCs expressed the typical MSC marker proteins CD13, CD29, and CD44 and differentiated into adipocytes, osteoblasts, and chondrocytes when exposed to the appropriate differentiation media. In addition, AF-MSC-conditioned media (AF-MSC-CM) significantly enhanced proliferation of dermal fibroblasts. Antibody-based protein array and enzyme-linked immunosorbent assay (ELISA) indicated that AF-MSC-CM contains various cytokines and chemokines that are known to be important in normal wound healing, including IL-8, IL-6, TGF-beta, TNFRI, VEGF, and EGF. Application of AF-MSC-CM significantly enhanced wound healing by dermal fibroblasts via the TGF-beta/SMAD2 pathway. Levels of p-SMAD2 were increased by AF-MSC-CM, and both the increase in p-SMAD2 and migration of dermal fibroblasts were blocked by inhibiting the TGF-beta/SMAD2 pathway. Moreover, in a mouse excisional wound model, AF-MSC-CM accelerated wound healing. These data provide the first evidence of the potential for AF-MSC-CM in the treatment of skin wounds.
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- Appears in
Collections - 2. Clinical Science > Department of Pathology > 1. Journal Articles
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