Gaegurin-6 stimulates insulin secretion through calcium influx in pancreatic β Rin5mf cells
- Authors
- Kim, Ji Hae; Lee, Jung Ok; Jung, Jin Hee; Lee, Soo Kyung; You, Ga Young; Park, Sun Hwa; Kim, Hyeon Soo
- Issue Date
- Jan-2010
- Publisher
- Elsevier BV
- Keywords
- Calcium; Gaegurin-6; Insulin; Pancreas
- Citation
- Regulatory Peptides, v.159, no.1-3, pp 123 - 128
- Pages
- 6
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Regulatory Peptides
- Volume
- 159
- Number
- 1-3
- Start Page
- 123
- End Page
- 128
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/15205
- DOI
- 10.1016/j.regpep.2009.07.014
- ISSN
- 0167-0115
1873-1686
- Abstract
- Gaegurin-6, an antimicrobial peptide that belongs to the alpha-helix family, was isolated from the skin of Rana rugosa. Gaegurin-6 contains a hydrophobic motif at the N-terminus and a helical region at the C-terminus. Although gaegurin-6 has been implicated in cell signaling, the precise role in insulin secretion is currently unknown. We have attempted to determine whether gaegurin-6 affects insulin secretion and tried to elucidate the relationship between the structural motifs and biological activity. In this study, we have shown that gaegurin-6 stimulates insulin secretion and also increases the intracellular calcium concentration in pancreatic beta Rin5mf cells. Moreover, a corollary study revealed that both the hydrophobicity of the N-terminus and the disulfide bridge of the C-terminus of gaegurin-6 are critical for its effects on insulin secretion. Membrane pore-forming ability is also observed in gaegurin-6, but not in the linear form or the N-terminus hydrophobic amino acid-deleted form. We further showed that these regions of gaegurin-6 are responsible for calcium influx in pancreatic beta Rin5mf cells. Taken together, these results indicate that gaegurin-6 can affect insulin secretion in pancreatic beta cells through the modulation of calcium influx. (C) 2009 Elsevier B.V. All rights reserved.
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