Intermolecular cross-talk between NTR1 and NTR2 neurotensin receptor promotes intracellular sequestration and functional inhibition of NTR1 receptors
- Authors
- Hwang, Jae Ryoung; Bael, Min Woo; Sim, Jeonggu; Choi, Heung-Sik; Han, Ji Man; Kim, You Lim; Hwang, Jong-Ik; Kwon, Hyuk Bang; Beaudet, Nicolas; Sarret, Philippe; Seong, Jae Young
- Issue Date
- 1-Jan-2010
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Neurotensin; G-protein-coupled receptor; Dimerization; Trafficking; Signal transduction
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.391, no.1, pp 1007 - 1013
- Pages
- 7
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 391
- Number
- 1
- Start Page
- 1007
- End Page
- 1013
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/15208
- DOI
- 10.1016/j.bbrc.2009.12.007
- ISSN
- 0006-291X
1090-2104
- Abstract
- G-protein-coupled receptors (GPCR) are now, regarded as being able to acquire heterodimer conformations affecting their pharmacology. signaling and trafficking in co-immunoprecipitation Studies using differentially epitope-tagged receptors. we herein provide direct evidence for heterodimerization of human neurotensin type 1 receptor (hNTR1) and type 2 receptor (hNTR2) Using chimeric constructs. we also identified the hNTR2 transmembrane 2 (TM2) to TM4 region as crucial for the formation of the dimerization interface. At the functional level, we demonstrated that the co-expression of hNTR2 suppressed hNTR1-mediated adenylate cyclase/cAMP and phospholipase C activation Finally, confocal microscopy revealed that whereas tagged hNTR1 expressed alone were localized to the plasma membrane. co-expression of hNTR2 caused the retention of hNTR1 in sub-cellular compartments, indicating that heterodimerization with hNTR2 interferes with the proper recruitment of hNTR1 to the plasma membrane Overall. this study proposes a novel function of NTR2 in the regulation of NTR1 activity (C) 2009 Elsevier Inc All rights reserved
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Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
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