O2 plasma patterning of p-type MWCNT and its application to immunosensor
- Authors
- Lee J.-Y.; Park E.J.; Kim J.H.; Kim S.G.; Lee C.J.; Kim M.J.; Min N.K.
- Issue Date
- Sep-2010
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- ELISA; Immunosensors; Legionella pneumophila; MWCNTs; Plasma-pattern
- Citation
- Thin Solid Films, v.518, no.22, pp 6646 - 6649
- Pages
- 4
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Thin Solid Films
- Volume
- 518
- Number
- 22
- Start Page
- 6646
- End Page
- 6649
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/15467
- DOI
- 10.1016/j.tsf.2009.12.107
- ISSN
- 0040-6090
- Abstract
- An electrochemical immunosensor based on patterned plasma-functionalized multiwalled carbon nanotubes (pf-MWCNTs) working electrode for Legionella pneumophila detection is described in this paper. Polyclonal antibody was immobilized using passive adsorption and covalent immobilization on the surface of the pf-MWCNTs. Various dilutions of Legionella 19-kDa peptidoglycan- associated lipoprotein (PAL) were added onto the electrode surface and incubated. The assay was then completed using polyclonal antibody conjugated to horseradish peroxidase (HRP) as the enzyme label. 3,3′,5,5′- Tetramethylbenzidine dihydrochloride (TMB)/substrate solution (H 2O2) was used as the mediator/substrate system. Electrochemical detection was conducted using cyclic voltammetric at 500 mV versus silver/silver chloride (Ag/AgCl) reference electrode. L. pneumophila detection of 0.16 ng ml- 1 and ∼ 0.0025 ng ml- 1 was achieved respectively for passive adsorption and covalent immobilization. © 2010 Elsevier B.V. All rights reserved.
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- Appears in
Collections - 2. Clinical Science > Department of Infectious Diseases > 1. Journal Articles
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