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Cited 34 time in webofscience Cited 35 time in scopus
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Formation of vitamin A lipid droplets in pancreatic stellate cells requires albumin

Authors
Kim, N.Yoo, W.Lee, J.Kim, H.Lee, H.Kim, Y-SKim, D-UOh, J.
Issue Date
Oct-2009
Publisher
BMJ PUBLISHING GROUP
Keywords
albumin; fibrosis; hepatic stellate cells; pancreatic stellate cells; vitamin A
Citation
GUT, v.58, no.10, pp.1382 - 1390
Indexed
SCIE
SCOPUS
Journal Title
GUT
Volume
58
Number
10
Start Page
1382
End Page
1390
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/15751
DOI
10.1136/gut.2008.170233
ISSN
0017-5749
Abstract
Objective: Quiescent pancreatic stellate cells (PSCs) store vitamin A as cytoplasmic lipid droplets, and, when activated by profibrogenic stimuli, they transform into myofibroblast-like cells characterised by the loss of vitamin A droplets. Activation of stellate cells is central to fibrogenesis, but the mechanism for the formation of vitamin A droplets and its relationship to stellate cell activation remain unclear. Methods: With use of cultured PSCs, an attempt was made to characterise the function of albumin endogenously expressed in stellate cells. Results: Albumin is endogenously expressed in quiescent PSCs, localised in cytoplasmic lipid droplets, and its levels are markedly reduced after stellate cell activation. Continuous albumin expression in stellate cells is sufficient to maintain their fat-storing phenotype even after cell passages and renders cells resistant to the activating effects of transforming growth factor beta (TGF beta). Forced expression of albumin in PSCs after passage 2 (activated PSCs) induced the re-appearance of lipid droplets and phenotypic changes, which were previously reported with retinol treatment. Retinol increases albumin synthesis in activated PSCs and the suppression of albumin expression using small interfering RNA (siRNA) abolishes retinol-induced effects. Conclusions: The data demonstrate a novel role for albumin in the formation of cytoplasmic vitamin A lipid droplets in stellate cells, and suggest that albumin may have a direct influence on stellate cell activation.
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3. Graduate School > Biomedical Research Center > 1. Journal Articles
2. Clinical Science > Department of Pathology > 1. Journal Articles
2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles

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Lee, Hong Sik
안암병원 (Department of Gastroenterology and Hepatology, Anam Hospital)
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