Retinoic Acid Leads to Cytoskeletal Rearrangement through AMPK-Rac1 and Stimulates Glucose Uptake through AMPK-p38 MAPK in Skeletal Muscle Cellsopen access
- Authors
- Lee, Yun Mi; Lee, Jung Ok; Jung, Jin-Hee; Kim, Ji Hae; Park, Sun-Hwa; Park, Ji Man; Kim, Eung-Kyun; Suh, Pann-Ghill; Kim, Hyeon Soo
- Issue Date
- 5-Dec-2008
- Publisher
- American Society for Biochemistry and Molecular Biology Inc.
- Citation
- Journal of Biological Chemistry, v.283, no.49, pp 33969 - 33974
- Pages
- 6
- Indexed
- SCOPUS
- Journal Title
- Journal of Biological Chemistry
- Volume
- 283
- Number
- 49
- Start Page
- 33969
- End Page
- 33974
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/16630
- DOI
- 10.1074/jbc.M804469200
- ISSN
- 0021-9258
1083-351X
- Abstract
- Retinoic acid (RA) is one of the major components of vitamin A. In the present study, we found that retinoic acid activated AMP-activated protein kinase (AMPK). RA induced Rac1-GTP formation and phosphorylation of its downstream target, p21-activated kinase (PAK), whereas the inhibition of AMPK blocked RA-induced Rac1 activation. Moreover, cofilin, an actin polymerization regulator, was activated when incubated with RA. We then showed that inhibition of AMPK by compound C, a selective inhibitor of AMPK, or small interfering RNA of AMPK alpha 1 blocked RA-induced cofilin phosphorylation. Additionally, we found that retinoic acid-stimulated glucose uptake in differentiated C2C12 myoblast cells and activated p38 mitogen-activated protein kinase (MAPK). Finally, the inhibition of AMPK and p38 MAPK blocked retinoic acid-induced glucose uptake. In summary, our results suggest that retinoic acid may have cytoskeletal roles in skeletal muscle cells via stimulation of the AMPK-Rac1-PAK-cofillin pathway and may also have beneficial roles in glucose metabolism via stimulation of the AMPK-p38 MAPK pathway.
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- Appears in
Collections - 4. Research institute > Institute of Human Genetics > 1. Journal Articles
- 1. Basic Science > Department of Anatomy > 1. Journal Articles
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