Serum ferritin and transferrin levels as serologic markers of methylene diphenyl diisocyanate-induced occupational asthma
- Authors
- Hur G.-Y.; Choi G.-S.; Sheen S.-S.; Lee H.-Y.; Park H.-J.; Choi S.-J.; Ye Y.-M.; Park H.-S.
- Issue Date
- 2008
- Keywords
- ferritin; MDI; occupational asthma; proteome; transferrin
- Citation
- Journal of Allergy and Clinical Immunology, v.122, no.4, pp 774 - 780
- Pages
- 7
- Indexed
- SCOPUS
- Journal Title
- Journal of Allergy and Clinical Immunology
- Volume
- 122
- Number
- 4
- Start Page
- 774
- End Page
- 780
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/17476
- DOI
- 10.1016/j.jaci.2008.07.034
- ISSN
- 0091-6749
1097-6825
- Abstract
- Background: Although methylene diphenyl diisocyanate (MDI) may induce occupational asthma in the workplace, the pathogenic mechanisms are unclear. Objectives: By using bronchoalveolar lavage fluid, we sought to identify proteins that were differentially expressed between subjects with MDI-induced occupational asthma (MDI-OA) and asymptomatic exposed controls (AECs). Methods: To find proteins that were differentially expressed between the MDI-OA and AEC groups, 2-dimensional electrophoresis was performed by using bronchoalveolar lavage fluid obtained from subjects after MDI-specific inhalation challenge. The selected protein spots were then identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The clinical relevance of the differentially expressed spots was compared by ELISA using sera from the MDI-OA/eosinophilic bronchitis, AEC, and unexposed healthy control groups. Receiver operating characteristic curves were then plotted, and the sensitivity and specificity were determined. Results: Twenty-three protein spots were identified that distinguished the subjects with MDI-OA from those in the AEC group. Among them, ferritin expression was downregulated whereas transferrin expression was upregulated in subjects with MDI-OA compared with AEC; these results were validated by ELISA using sera from the MDI-OA/EB and AEC groups. To identify subjects with MDI-OA, the optimal serum cutoff levels were 69.84 ng/mL for ferritin and 2.48 μg/mL for transferrin. When these 2 parameters were combined, the sensitivity was 71.43% and the specificity was 85.71%. Conclusion: Serum ferritin and transferrin levels are associated with the phenotype of MDI-OA. © 2008 American Academy of Allergy, Asthma & Immunology.
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- Appears in
Collections - 2. Clinical Science > Department of Pulmonary, Allergy, and Critical Care Medicine > 1. Journal Articles
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