Vascular endothelial growth factor induces endothelin-1 production via matrix metalloproteinase-2 rather than endothelin-converting enzyme-1
- Authors
- Lee, Kyung-Ju; Kim, Meyoung-Kon; Park, Yoon-Hee; Seol, Hyun-Joo; Lim, Ji-Eun; Lee, Jeong No; Oh, Min-Jeong
- Issue Date
- 2007
- Publisher
- TAYLOR & FRANCIS INC
- Keywords
- vascular endothelial growth factor; endothelin-converting enzyme-1; matrix metalloproteinase-2; endothelin-1; preeclampsia
- Citation
- HYPERTENSION IN PREGNANCY, v.26, no.2, pp 189 - 199
- Pages
- 11
- Indexed
- SCIE
SCOPUS
- Journal Title
- HYPERTENSION IN PREGNANCY
- Volume
- 26
- Number
- 2
- Start Page
- 189
- End Page
- 199
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18438
- DOI
- 10.1080/10641950701204604
- ISSN
- 1064-1955
1525-6065
- Abstract
- Objective: To investigate the mechanism of vascular endothelial growth factor (VEGF)-induced endothelin-1 production in human umbilical vein endothelial cells (HUVECs). Methods: Endothelin-1 levels were measured in conditioned medium of women with preeclampsia HUVECs were treated with different concentrations of VEGF(165) and at various time intervals. Next, we measured endothelin-1 levels after HUVECs were also incubated with VEGF and endothelin-converting enzyme-1 (ECE-1) inhibitor or tissue inhibitors of matrix metalloproteinase-2 (TIMP-2). Additionally, the circulating levels of total and free VEGF, matrix metalloproteinase-2 (MMP-2), and endothelin-1 were measured in 20 preeclamptic patients and 20 healthy pregnant controls. Results: HUVECs treated with VEGF increased their endothelin-1 production in a concentration and time-dependent manner. The production of endothelin-1 was inhibited by TIMP-2, but not by the ECE-1 inhibitor. Total VEGF, MMP-2, and endothelin-1 concentrations were higher in preeclampsia and showed significant positive correlations between them. Conclusion: These findings suggest that VEGF-induced endothelin-1 production might be mediated by MMP-2 rather than by ECE-1 upregulation.
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- Appears in
Collections - 2. Clinical Science > Department of Obstetrics and Gynecology > 1. Journal Articles
- 1. Basic Science > Department of Biochemistry and Molecular Biology > 1. Journal Articles
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