플라즈마와 세포외기질 단백질을 처리한 PLA 표면에서의 인간 중간엽 줄기세포의 부착과 증식평가

Abstract

Poly(L-lactic acid) (PLLA) is biodegradable polymer and has been widely used as a basic material for scaffold in tissue engineering. Cell adhesion and proliferation on the polymer scaffold surface is one of the important issues and this is influenced by surface characteristics of polymer scaffolds. In this study, PLLA surface was modified by plasma treatment and/or coating of extracellular matrix (ECM) protein. Human mesenchymal stem cells (hMSCs) were cultured on the different PLLA surfaces and cellular activity and adhesion were measured quantitatively. PLLA was activated by O2 plasma and the different levels of O2 plasma were applied to each PLLA group to change the surface characteristics. The hydrophobicity of O2 plasma-treated PLLA surface was measured by water contact angle and the surface characteristics of samples were analyzed by Fourier transform infrared spectroscopy (FT-IR), x-ray photoelectron spectroscopy (XPS) and atomic force microscope (AFM). Each ECM protein (collagen, fibronectin, vitronectin) was also coated on the non-treated or plasma-treated PLLA surface to prove the effect of extracellular matrix protein. hMSCs were cultured on different samples (PLLA±plasma, plasma-treated PLLA±ECM protein) to evaluate the cell adhesion and proliferation. The proliferation of hMSCs was analyzed by WST-1 test and the adhesion of hMSCs was quantified by image analyzing software. Coating of ECM protein on the surface increased the hMSCs adhesion and proliferation in spite of the change of hydrophobicity. The spreading of hMSCs decreased on the plain PLLA surface, whereas mouse fibroblast spreaded at the same condition. Cells on collagen coated, plasmatreated PLLA showed highest cell adhesion among samples. This study provides basic clues for cell adhesion and remodeling of scaffold by hMSCs in PLLA based scaffolds.