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Cited 3 time in webofscience Cited 4 time in scopus
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FxClear, A Free-hydrogel Electrophoretic Tissue Clearing Method for Rapid De-lipidation of Tissues with High Preservation of Immunoreactivity

Authors
Choi, JungyoonLee, EunsooKim, June HoanSun, Woong
Issue Date
Jun-2019
Publisher
KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE
Keywords
Three dimensional imaging; Immunohistochemistry; Tissue engineering; Brain tissue
Citation
EXPERIMENTAL NEUROBIOLOGY, v.28, no.3, pp.436 - 445
Indexed
SCIE
SCOPUS
KCI
Journal Title
EXPERIMENTAL NEUROBIOLOGY
Volume
28
Number
3
Start Page
436
End Page
445
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/1994
DOI
10.5607/en.2019.28.3.436
ISSN
1226-2560
Abstract
Over the last two decades, several tissue clearing methodologies have been established that render tissues optically transparent and allow imaging of unsectioned tissues of significant volumes, thus improving the capacity to study the relationships between cell and 3D tissue architecture. Despite these technical advances, the important unsolved challenges that these methods face include complexity, time, consistency of tissue size before and after clearing, and ability to immunolabel various antibodies in cleared tissue. Here, we established very simple and fast tissue clearing protocol, FxClear, which involves acrylainide- free electrophoretic tissue clearing (ETC). By removal of the acrylamide infusion step, we were able to achieve fast reaction time, smaller tissue expansion, and higher immunoreactivity. Especially, immunoreactivity and fluorescence intensity were increased in FxClear-processed tissues compared to un-cleared tissues. Our protocol may be suitable for small-sized biopsy samples for 3D pathological examinations.
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