Immunochemical and biological analysis of allergenicity with excretory-secretory products of Anisakis simplex third stage larva
- Authors
- Kim, JS; Kim, KH; Cho, S; Park, HY; Cho, SW; Kim, YT; Joo, KH; Lee, JS
- Issue Date
- 2005
- Publisher
- KARGER
- Keywords
- allergen; Anisakis simplex; mast cells; ELISA; IgE; Western blot
- Citation
- INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, v.136, no.4, pp 320 - 328
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
- Volume
- 136
- Number
- 4
- Start Page
- 320
- End Page
- 328
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/20180
- DOI
- 10.1159/000084225
- ISSN
- 1018-2438
1423-0097
- Abstract
- Background: Anisakis simplex third stage larvae (L3) are parasites that frequently give rise to allergic responses. The larvae molt into fourth stage larvae (L4), and at each stage they produce L3-excretory-secretory products (L3-ESP) and L4-ESP, respectively, which are different in their main protein constituents. Although the allergenicity of L4-ESP has been investigated by several research groups, research on the allergenicity of L3-ESP has not been carried out by any researcher. In this investigation, the allergenicity and antigenicity of L3-ESP were investigated in comparison with L4-ESP, using rat sera. Methods: Rat sera were produced by L3 oral infection two times with a 9-week interval. Larvae ESP prepared by culture were concentrated and fractioned using lyophilizer and a centrifugal filter device, respectively. Immunochemical analysis was performed using both indirect ELISA and immunoblot. Biological allergenicity was analyzed by RBL-2H3 exocytosis. Results: With the indirect ELISA, the optical density (OD) value of the nonfractioned (NF)-L3ESP was only one third of that of the NF-L4ESP in both specific IgM and IgG. On measuring specific IgE, the OD of NF-L3ESP was less than one tenth of that of NF-L4ESP. In addition, neither antigen nor allergen was shown in NF-L3ESP, but it was shown in NF-L4ESP with immunoblot. However, the biological allergenicity of NF-L3ESP was comparable to that of NF-L4ESP. To demonstrate the presence of any allergen, L3-ESP was fractioned and found to carry twelve visualized allergen bands from 10 to 186 kDa by immunoblot. Conclusions: These results indicate that L3-ESP may include the important allergens necessary to induce the allergy by L3 oral infection, as compared to L4-ESP. Copyright (C) 2005 S. Karger AG, Basel.
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Collections - 1. Basic Science > Department of Parasitology > 1. Journal Articles
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