Vaccination with a DNA Vaccine Encoding Herpes Simplex Virus Type 1 VP22 Linked to Antigen Generates Long-Term Antigen-Specific CD8-Positive Memory T Cells and Protective Immunity
- Authors
- Kim T.W.; Hung C.-F.; Kim J.W.; Juang J.; Chen P.-J.; He L.; Boyd D.A.K.; Wu T.-C.
- Issue Date
- 2004
- Publisher
- Mary Ann Liebert Inc.
- Citation
- Human Gene Therapy, v.15, no.2, pp 167 - 177
- Pages
- 11
- Indexed
- SCOPUS
- Journal Title
- Human Gene Therapy
- Volume
- 15
- Number
- 2
- Start Page
- 167
- End Page
- 177
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/20873
- DOI
- 10.1089/104303404772679977
- ISSN
- 1043-0342
1557-7422
- Abstract
- Intradermal vaccination with DNA encoding herpes simplex virus type 1 (HSV-1) VP22 linked to antigen leads to spread of antigen within the epithelium and results in enhanced antigen-specific CD8+ T cell immune responses in vaccinated mice. In this study, we characterized the number of antigen-expressing dendritic cells (DCs) in the draining lymph nodes of vaccinated mice and determined whether the linkage of VP22 to antigen would influence the ability of antigen-expressing DCs to activate antigen-specific CD8+ T cells in vivo. Vaccination with DNA encoding HSV-1 VP22 linked to human papillomavirus type 16 E7 antigen generated more antigen-expressing DCs in the draining lymph nodes of vaccinated mice than E7 alone. In addition, the linkage of VP22 to E7 improved the MHC class I presentation of E7 in transfected DCs and led to enhanced activation of E7-specific CD8+ T cells. We also observed that vaccination with DNA encoding VP22 linked to E7 generated more E7-specific CD8+ memory T cells, and enhanced long-term protective antitumor immunity against an E7-expressing tumor in vaccinated mice compared with vaccination with DNA encoding E7 alone. Thus, administration of DNA encoding VP22 linked to antigen represents a plausible approach for the development of protective DNA vaccines.
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Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
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