Differential desensitization and internalization of three different bullfrog gonadotropin-releasing hormone receptors
- Authors
- Acharjee, S; Maiti, K; Soh, JM; Im, WB; Seong, JY; Kwon, HB
- Issue Date
- 31-Aug-2002
- Publisher
- SPRINGER-VERLAG SINGAPORE PTE LTD
- Keywords
- beta-arrestin; bullfrog; desensitization; dynamin; gonadotropin-releasing hormone receptor (GnRHR); internalization
- Citation
- MOLECULES AND CELLS, v.14, no.1, pp 101 - 107
- Pages
- 7
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MOLECULES AND CELLS
- Volume
- 14
- Number
- 1
- Start Page
- 101
- End Page
- 107
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/21760
- ISSN
- 1016-8478
0219-1032
- Abstract
- We previously demonstrated the presence of three distinct types of the gonadotropin-releasing hormone receptor (GnRHR) in a bullfrog (denoted bfGnRHR-1, bfGnRHR-2, and bfGnRHR-3). The bfGnRHRs exhibited differential tissue distribution and ligand selectivity. In the present study, we demonstrated the desensitization and internalization kinetics of these receptors in both transiently-transfected HEK293 cells and retrovirus-mediated stable cells. The time-course accumulation of the inositol phosphate in response to GnRH revealed that bfGnRHR-1 and -2 were rapidly desensitized, whereas bfGnRHR-3 was slowly desensitized. A comparison of the internalization kinetics revealed the most rapid rate and highest extent of internalization of bfGnRHR-2 among the three receptors. Interestingly, the mechanisms that underlie the receptor internalization appear to differ from each other. Internalization of bfGnRHR-1 was dependent on both dynamin and P-arrestin, whereas those of bfGnRHR-2 and -3 were dependent on dynamin, but not on beta-arrestin. These results, therefore, suggest that differential regulatory mechanisms for desensitization and internalization of the GnRHR are involved in diverse cellular and physiological responses to GnRH stimulation.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.