Analysis of steroid-induced genes in the rat preoptic area-anterior hypothalamus using a differential-display reverse transcriptase-polymerase chain reaction
- Authors
- Park, S; Seong, JY; Son, GH; Kang, SS; Lee, S; Kim, SR; Kim, K
- Issue Date
- Jun-2001
- Publisher
- WILEY
- Keywords
- steroids; genes; DD-PCR; POA-AH; puberty; oestrous cycle
- Citation
- JOURNAL OF NEUROENDOCRINOLOGY, v.13, no.6, pp 531 - 539
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF NEUROENDOCRINOLOGY
- Volume
- 13
- Number
- 6
- Start Page
- 531
- End Page
- 539
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/22517
- DOI
- 10.1046/j.1365-2826.2001.00665.x
- ISSN
- 0953-8194
1365-2826
- Abstract
- Steroid hormones modulate a variety of physiological functions in the hypothalamus. We attempted to identify steroid-regulated genes in the rat preoptic area-anterior hypothalamus by comparing differentially expressed mRNAs, Adult female rats were ovariectomized and, 1 week later, a silastic capsule containing 17 beta -oestradiol (180 mug/ml) was subcutaneously implanted. After 2 days, a single injection of progesterone (1 mg) was administered at 10.00 h and rats were killed at 17.00 h on the same day, Differential-display polymerase chain reaction followed by Northern blot analysis showed that 10 clones were differentially regulated. Using homology search in Genbank, three genes were identified as sodium, potassium-ATPase beta1, protein kinase C-binding Nell-homologue protein and evectin-1, Further characterization of 10 clones showed that the expression patterns were tissue-specific and differentially regulated during puberty. Among these, mRNAs for protein kinase C-binding Nell-homologue protein, evectin-1 and human CGI-118 protein-like gene were induced after vagina opening, and differentially expressed during the oestrous cycle. Taken together, several steroid-regulated genes identified in the present study may play an important role in regulating hypothalamic functions, including puberty and the oestrous cycle.
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Collections - 3. Graduate School > Biomedical Research Center > 1. Journal Articles
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