A high glucose concentration stimulates the expression of monocyte chemotactic peptide 1 in human mesangial cells
- Authors
- Ihm, CG; Park, JK; Hong, SP; Lee, TW; Cho, BS; Kim, MJ; Cha, DR; Ha, H
- Issue Date
- May-1998
- Publisher
- KARGER
- Keywords
- monocyte chemotactic peptide 1; high-glucose medium; mesangial cells; protein kinase C
- Citation
- NEPHRON, v.79, no.1, pp 33 - 37
- Pages
- 5
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEPHRON
- Volume
- 79
- Number
- 1
- Start Page
- 33
- End Page
- 37
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/24663
- DOI
- 10.1159/000044988
- ISSN
- 0028-2766
- Abstract
- The mechanism of glomerular infiltration of monocytes remains unknown in diabetic nephropathy. We examined the effect of a high glucose concentration on monocyte chemotactic peptide 1 (MCP-1) expression in human mesangial cells (MCs) by using enzyme-linked immunosorbent assay and reverse transcription coupled with polymerase chain reaction (PCR). More than a 50% increase in the MCP-1 protein production was observed in MCs cultured in high-glucose medium (450 mg/dl) as compared to normal glucose (100 mg/dl; 1,496 +/- 75 vs. 966 +/- 15 pg/ml after 24 h, 1,910 +/- 93 vs. 1,250 +/- 55 pg/ml after 48 h). Semiquantitative PCR showed that phorbol. myristate acetate (100 nM) increased the ratio of PCR products for MCP-1 to housekeeping gene glyceraldehyde-3-phosphate dehydrogenase on densitometric results at 24 h by 2.7-fold, which was prevented by calphostin C (200 nM) pretreatment. High glucose increased the ratio by 3-fold as compared to normal glucose at 24 h (0.72 +/- 0.11 vs. 0.24 +/- 0.01). This was also suppressed by calphostin C pretreatment. These findings demonstrate that high glucose can directly increase MCP-1 expression in MCs, which may contribute to monocyte infiltration in diabetic nephropathy, and this is regulated by protein kinase C.
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- Appears in
Collections - 2. Clinical Science > Department of Nephrology and Hypertension > 1. Journal Articles
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