Involvement of N-methyl-D-aspartate receptor and free radical in homocysteine-mediated toxicity on rat cerebellar granule cells in culture
- Authors
- Kim W.-K.; Pae Y.-S.
- Issue Date
- 1996
- Publisher
- Elsevier Ireland Ltd
- Keywords
- Cerebellar granule cell; Free radical; Homocysteine; Lactate dehydrogenase; N-Methyl-D-aspartate
- Citation
- Neuroscience Letters, v.216, no.2, pp 117 - 120
- Pages
- 4
- Indexed
- SCOPUS
- Journal Title
- Neuroscience Letters
- Volume
- 216
- Number
- 2
- Start Page
- 117
- End Page
- 120
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/26095
- DOI
- 10.1016/S0304-3940(96)13011-1
- ISSN
- 0304-3940
1872-7972
- Abstract
- The present study investigates the possible mechanism responsible for the neurotoxicity of D,L-homocysteine in primary culture of rat cerebellar granule cells. Neurotoxicity was assessed by measuring the amount of lactate dehydrogenase released from the cells following homocysteine treatment. D,L-Homocysteine (>300 μM; 16-22 h) induced the release of lactate dehydrogenase from the cells in a concentration-dependent manner. The N-methyl-D-aspartate (NMDA) antagonist (±)-2-amino-5-phosphonopentanoic acid (APV) partially blocked the homocysteine-mediated neurotoxicity. However, the non-NMDA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) did not block the homocysteine-mediated toxicity. The homocysteine-mediated neurotoxicity was mostly prevented by the co-administration of superoxide dismutase and catalase or catalase alone. The results suggest that homocysteine induces neuronal cell death by stimulating NMDA receptor as well as by producing free radicals.
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- Appears in
Collections - 1. Basic Science > Department of Neuroscience > 1. Journal Articles
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