BrainFilm, a novel technique for physical compression of 3D brain slices for efficient image acquisition and post-processing
- Authors
- Kim, Joo Yeon; Kim, Hyun Jung; Jang, Min Jee; Kim, June Hoan; Lee, Ju-Hyun; Lee, Eunsoo; Park, Kyerl; Kim, Hyuncheol; Lee, Jaedong; Kwag, Jeehyun; Kim, Namhee; Song, Mi-Ryoung; Kim, Hyun; Sun, Woong
- Issue Date
- Jun-2018
- Publisher
- Nature Publishing Group
- Citation
- Scientific Reports, v.8, no.1
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Scientific Reports
- Volume
- 8
- Number
- 1
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/3436
- DOI
- 10.1038/s41598-018-26776-9
- ISSN
- 2045-2322
- Abstract
- Tissue clearing enables us to observe thick tissue at a single cell resolution by reducing light scattering and refractive index matching. However, imaging of a large volume of tissue for 3D reconstruction requires a great deal of time, cost, and efforts. Few methods have been developed to transcend these limitations by mechanical compression or isotropic tissue shrinkage. Tissue shrinkage significantly lessens the imaging burden; however, there is an inevitable trade-off with image resolution. Here, we have developed the "BrainFilm" technique to compress cleared tissue at Z-axis by dehydration, without alteration of the XY-axis. The Z-axis compression was approximately 90%, and resulted in substantial reduction in image acquisition time and data size. The BrainFilm technique was successfully used to trace and characterize the morphology of thick biocytin-labelled neurons following electrophysiological recording and trace the GFP-labelled long nerve projections in irregular tissues such as the limb of mouse embryo. Thus, BrainFilm is a versatile tool that can be applied in diverse studies of 3D tissues in which spatial information of the Z-axis is dispensable.
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- Appears in
Collections - 1. Basic Science > Department of Anatomy > 1. Journal Articles
- 3. Graduate School > Graduate School > 1. Journal Articles
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