Duox2 is required for the transcription of pattern recognition receptors in acute viral lung infection: An interferon-independent regulatory mechanism
- Authors
- Hong, Seung-No; Kim, Ji Young; Kim, Hanna; Kim, Dong-Young; Won, Tae-Bin; Han, Doo Hee; Rhee, Chae-Seo; Kim, Hyun Jik
- Issue Date
- Oct-2016
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Influenza A virus; Duox2; RIG-I; MDA5; Acute viral lung infection
- Citation
- ANTIVIRAL RESEARCH, v.134, pp 1 - 5
- Pages
- 5
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- ANTIVIRAL RESEARCH
- Volume
- 134
- Start Page
- 1
- End Page
- 5
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/34420
- DOI
- 10.1016/j.antiviral.2016.08.017
- ISSN
- 0166-3542
1872-9096
- Abstract
- The innate immune response, which constitutes the first line of defense against influenza A virus (IAV) infection, is activated by pattern recognition receptors (PRRs) that recognize viral structures. We found that the PRRs, retinoic acid-inducible gene 1 (RIG-I) and melanoma differentiation-associated protein 5 (MDA5), which have been implicated as interferon (IFN)-stimulated genes, were dominantly responsible for the recognition of IAV in lungs of mice at 3 and 7 days post infection (dpi). Intranasal administration of IFNs enhanced RIG-I and MDA5 gene expression after IAV infection and mRNA levels of RIG-I and MDA5 were significantly reduced at 7 dpi in mice with neutralization of secreted IFNs. However, blockade of IFNs did not alter the transcription of RIG-I and MDA5 at 3 dpi. We studied the antiviral effect of Duox2 in vivo lung to elucidate the role of Duox2 in respiratory mucosa. RIG-I and MDA5 mRNA levels were induced to a lower extent in lungs of mice that were inoculated with Duox2 small hairpin RNA regardless of secreted IFNs at 3 dpi. We propose that Duox2 is responsible for IFN-independent signaling for induction of PRRs transcription and can control acute IAV lung infection at the beginning of infection. (C) 2016 Elsevier B.V. All rights reserved.
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