In Vitro Anti-Inflammation and Chondrogenic Differentiation Effects of Inclusion Nanocomplexes of Hyaluronic Acid-Beta Cyclodextrin and Simvastatinopen access
- Authors
- Kim, Tae-Hoon; Yun, Young-Pil; Shim, Kyu-Sik; Kim, Hak Jun; Kim, Sung Eun; Park, Kyeongsoon; Song, Hae-Ryong
- Issue Date
- Jun-2018
- Publisher
- 한국조직공학과 재생의학회
- Keywords
- Hyaluronic acid-beta-cyclodextrin; Simvastatin; Chondrogenesis; Adipose-derived stem cells
- Citation
- Tissue Engineering and Regenerative Medicine, v.15, no.3, pp 263 - 274
- Pages
- 12
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Tissue Engineering and Regenerative Medicine
- Volume
- 15
- Number
- 3
- Start Page
- 263
- End Page
- 274
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/3450
- DOI
- 10.1007/s13770-018-0119-9
- ISSN
- 1738-2696
2212-5469
- Abstract
- The aim of this study was to prepare inclusion nanocomplexes of hyaluronic acid-beta-cyclodextrin and simvastatin (HA-beta-CD/SIM) and evaluate in vitro anti-inflammation effects on lipopolysaccharide (LPS)-activated synoviocytes and chondrogenic differentiation effects on rat adipose-derived stem cells (rADSCs). The beta-CD moieties in HA-beta-CD could incorporate SIM to form HA-beta-CD/SIM nanocomplexes with diameters of 297-350 nm. HA-beta-CD/SIM resulted in long-term release of SIM from the nanocomplexes for up to 63 days in a sustained manner. In vitro studies revealed that HA-beta-CD/SIM nanocomplexes were able to effectively and dose-dependently suppress the mRNA expression levels of pro-inflammatory markers such as matrix metallopeptidase-3 (MMP-3), MMP-13, cyclooxygenase-2 (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS-5), interleukin-6 (IL-6), and tumor necrosis factor (TNF-alpha) in LPS-stimulated synoviocytes. HA-beta-CD/SIM-treated rADSCs significantly and dose-dependently enhanced mRNA expressions of aggrecan, collagen type II (COL2A1), and collagen type X (COL10A1), implying that HA-beta-CD/SIM greatly induced the chondrogenic differentiation of rADSCs. Conclusively, HA-beta-CD/SIM nanocomplexes will be a promising therapeutic material to alleviate inflammation as well as promote chondrogenesis.
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Collections - 4. Research institute > Nano-based Disease Control Institute > 1. Journal Articles
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