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Biological imaging of HEK293 cells expressing PLC gamma 1 using surface-enhanced raman microscopy
- Authors
- Lee, Sangyeop; Kim, Sungyong; Choo, Jaebum; Shin, Soon Young; Lee, Young Han; Choi, Ha Young; Ha, Seunghan; Kang, Kyungho; Oh, Chil Hwan
- Issue Date
- 1-Feb-2007
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.79, no.3, pp 916 - 922
- Pages
- 7
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 79
- Number
- 3
- Start Page
- 916
- End Page
- 922
- ISSN
- 0003-2700
1520-6882
- Abstract
- Surface-enhanced Raman scattering (SERS) imaging has been used for the targeting and imaging of specific cancer markers in live cells. For this purpose, Au/Ag core-shell nanoparticles, conjugated with monoclonal antibodies, were prepared. The procedures to label live cells with those bimetallic nanoprobes have been developed and used for highly sensitive SERS imaging of live cells. In the present study, live HEK293 cells expressing PLC gamma 1 have been used as the optical imaging target. Our results demonstrate the potential feasibility of SERS imaging technology for the highly sensitive imaging of cancer biomarkers in live cells.
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Related Researcher
- Kang, Kyung Ho
- Guro Hospital (Department of Pulmonary, Allergy, and Critical Care Medicine, Guro Hospital)