Osmosensitive gene expression of taurine transporter and cyclin C in embryonic fibroblast cells
- Authors
- Oh C.; Choi Y.J.; Kim H.G.; Lee D.-H.
- Issue Date
- 2006
- Publisher
- Springer New York
- Keywords
- Cyclin; Cysteine dioxygenase; Hypertonicity; Taurine
- Citation
- Advances in Experimental Medicine and Biology, v.583, pp 49 - 57
- Pages
- 9
- Indexed
- SCOPUS
- Journal Title
- Advances in Experimental Medicine and Biology
- Volume
- 583
- Start Page
- 49
- End Page
- 57
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/36191
- DOI
- 10.1007/978-0-387-33504-9_5
- ISSN
- 0065-2598
2214-8019
- Abstract
- Hypertonic conditions induce osmoregulatory activity. Molecular mechanism, however, remains to be further elucidated concerning the osmosensitive balancing activity. Using a differential display protocol, the genes of taurine transporter (TauT) and cyclin C were identified as candidate responding to the hypertonicity. When a quantitative PCR analysis was performed on the total RNA from KBEF cell treated under hypertonic conditions, the gene expressions of TauT and cyclin C were greatly increased. In terms of protein, the level of TauT expression increased up to 3.2-fold in response to the hypertonic treatment. Similarly to TauT, cyclin C protein also increased 2.4-fold compared to the control treatments. Under taurine-rich extracellular conditions, however, the level of TauT expression increased as little as 1.8-fold by hypertonicity treatment. Cyclin C expression was also lowered compared with low-taurine hypertonic treatment. Cysteine dioxygenase was shown to be highly responsive to the hypertonic treatment. Taken together, these results strongly indicate that the gene expressions of TauT and cyclin C are cooperatively regulated under hypotonic conditions. © 2006 Springer Science+Business Media, LLC.
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