하기도 감염 환아에서 분리된 Adenovirus 1, 2, 5 혈청형의 유전체형 분석Genome Type Analysis of Adenovirus Serotypes 1, 2 and 5 Isolated from Children with Lower Respiratory Tract Infections in Korea
- Other Titles
- Genome Type Analysis of Adenovirus Serotypes 1, 2 and 5 Isolated from Children with Lower Respiratory Tract Infections in Korea
- Authors
- 박기원; 최은화; 정지태; 이환종; 박기호
- Issue Date
- 2005
- Publisher
- 대한소아감염학회
- Keywords
- Adenovirus; Genome type; Serotypes; Molecular epidemiology
- Citation
- Pediatric Infection and Vaccine, v.12, no.2, pp 166 - 177
- Pages
- 12
- Journal Title
- Pediatric Infection and Vaccine
- Volume
- 12
- Number
- 2
- Start Page
- 166
- End Page
- 177
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/36411
- ISSN
- 2384-1079
2384-1087
- Abstract
- Pur po se : The purpose of this study was to examine the molecular epidemiology and
genetic variability of adenovirus(Ad) serotypes Ad1, Ad2, and Ad5 over 14 years in Korea.
Methods : A total of 382 adenoviral strains isolated from the nasopharyngeal aspirates of
children with lower respiratory tract infections in Seoul, Korea from November 1990 to February
2003 were serotyped by neutralization assay with type-specific antisera. Viral DNAs
were extracted from infected cell lysates by the modified Hirt procedure. Genome type(GT)
was determined by DNA restriction analysis with 12 restriction enzymess(BamHI, BclI, BglI,
BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmaI, XbaI, and XhoI). To evaluate the genetic
relatedness, pairwise comigrating restriction fragments(PCRF) analysis was performed.
Results : Of 382 strains, 33 strains(9%) were Ad1, 45 strains(12%) were Ad2, and 24
strains(6%) were Ad5. Eighteen GTs(Ad1p1-Ad1p7, Ad1a, Ad1b, Ad1b1-Ad1b3, Ad1c, Ad1d,
Ad1e, Ad1e1, Ad1e2, Ad1f) among Ad1, 24(Ad2p1-Ad2p11, Ad2a, Ad2a1-Ad2a6, Ad2b,
Ad2c, Ad2d, Ad2e, Ad2e1-Ad2e3) among Ad2, and 10(Ad5p1, Ad5p2, Ad5a, Ad5a1-Ad5a7)
among Ad5 strains were identified. One or two strains of the vast majority of GTs were isolated
during the study period while a few GTs were identified sporadically with more than 2
strains. It is notable that some GTs such as Ad1p5 and Ad5a1 appeared in cluster during a
short period. In analysis of genetic relatedness, the degree of PCRFs(pairwise comigrating restriction fragments) for Ad1 varied from 79 to 99%, for Ad2, 82 to 99%, and for Ad5, 85
to 99%.
Co nclusio n : This study established the comprehensive nomenclature systems of Ad1,
Ad2, and Ad5. Diverse GTs identified in this study have crucial implications in the genomic
diversity and epidemiological characteristics of Ad1, Ad2, and Ad5.
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Collections - 2. Clinical Science > Department of Pediatrics > 1. Journal Articles
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