Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates
- Authors
- Cui, Long-Hui; Joo, Hyung Joon; Kim, Dae Hwan; Seo, Ha-Rim; Kim, Jung Suk; Choi, Seung-Cheol; Huang, Li-Hua; Na, Ji Eun; Lim, I-Rang; Kim, Jong-Ho; Rhyu, Im Joo; Hong, Soon Jun; Lee, Kyu Back; Lim, Do-Sun
- Issue Date
- Jan-2018
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Extracellular matrix; Gradient nanopattern plates; Human endothelial colony-forming cells; Focal adhesion; Rho-associated protein kinase
- Citation
- ACTA BIOMATERIALIA, v.65, pp 272 - 282
- Pages
- 11
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- ACTA BIOMATERIALIA
- Volume
- 65
- Start Page
- 272
- End Page
- 282
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/4055
- DOI
- 10.1016/j.actbio.2017.10.026
- ISSN
- 1742-7061
1878-7568
- Abstract
- Nanotopography plays a pivotal role in the regulation of cellular responses. Nonetheless, little is known about how the gradient size of nanostructural stimuli alters the responses of endothelial progenitor cells without chemical factors. Herein, the fabrication of gradient nanopattern plates intended to mimic microenvironment nanotopography is described. The gradient nanopattern plates consist of nanopillars of increasing diameter ranges [120-200 nm (GP 120/200), 200-280 nm (GP 200/280), and 280-360 nm (GP 280/360)] that were used to screen the responses of human endothelial colony-forming cells (hECFCs). Nanopillars with a smaller nanopillar diameter caused the cell area and perimeter of hECFCs to decrease and their filopodial outgrowth to increase. The structure of vinculin (a focal adhesion marker in hECFCs) was also modulated by nanostructural stimuli of the gradient nanopattern plates. Moreover, Rho-associated protein kinase (ROCK) gene expression was significantly higher in hECFCs cultured on GP 120/200 than in those on flat plates (no nanopillars), and ROCK suppression impaired the nanostructural-timuli-induced vinculin assembly. These results suggest that the gradient nanopattern plates generate size-specific nanostructural stimuli suitable for manipulation of the response of hECFCs, in a process dependent on ROCK signaling. This is the first evidence of size-specific nanostructure-sensing behavior of hECFCs. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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- Appears in
Collections - 1. Basic Science > Department of Anatomy > 1. Journal Articles
- 4. Research institute > Cardiovascular Research Institute > 1. Journal Articles
- 3. Graduate School > Graduate School > 1. Journal Articles
- 4. Research institute > Metabolic Syndrome Research Center > 1. Journal Articles
- 2. Clinical Science > Department of Cardiology > 1. Journal Articles
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