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Cited 24 time in webofscience Cited 25 time in scopus
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Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord

Authors
Zhang, JunChandrasekaran, GopalakrishnanLi, WentingKim, Dong-YoungJeong, In YoungLee, So-HyunLiang, TingBae, Jin YoungChoi, IsaacKang, HyunoMaeng, Jin-SooKim, Myeong-KyuLee, TaewonPark, Seung WooKim, Min JungKim, Hyung-SeokRo, HyunjuBae, Yong ChulPark, Hae-ChulChoi, Eun YoungChoi, Seok-Yong
Issue Date
Apr-2020
Publisher
Nature Publishing Group
Citation
Nature Communications, v.11, no.1
Indexed
SCIE
SCOPUS
Journal Title
Nature Communications
Volume
11
Number
1
URI
https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/49356
DOI
10.1038/s41467-020-15248-2
ISSN
2041-1723
Abstract
Ependymal cells (ECs) are multiciliated neuroepithelial cells that line the ventricles of the brain and the central canal of the spinal cord (SC). How ependymal motile cilia are maintained remains largely unexplored. Here we show that zebrafish embryos deficient in Wnt signaling have defective motile cilia, yet harbor intact basal bodies. With respect to maintenance of ependymal motile cilia, plc delta 3a is a target gene of Wnt signaling. Lack of Connexin43 (Cx43), especially its channel function, decreases motile cilia and intercellular Ca2+ wave (ICW) propagation. Genetic ablation of cx43 in zebrafish and mice diminished motile cilia. Finally, Cx43 is also expressed in ECs of the human SC. Taken together, our findings indicate that gap junction mediated ICWs play an important role in the maintenance of ependymal motile cilia, and suggest that the enhancement of functional gap junctions by pharmacological or genetic manipulations may be adopted to ameliorate motile ciliopathy. Ependymal cells are supporting cells in the central nervous system. Here the authors elucidate a signalling axis in zebrafish spinal cord ependymal cells that is important for motile cilia assembly and maintenance, demonstrating that it depends on intercellular propagation of calcium ions via connexin 43.
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