Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma
- Authors
- Jung, Jik-Han; Back, Woojin; Yoon, Junyong; Han, Hyeonjeong; Kang, Ka -Won; Choi, Byeong Hyeon; Jeong, Hyesun; Park, Jaena; Shin, Hyunku; Hur, Woojune; Choi, Yeonho; Hong, Sunghoi; Kim, Hyun Koo; Park, Yong; Park, Ji-Ho
- Issue Date
- 12-Jan-2021
- Publisher
- Nature Research
- Citation
- Scientific Reports, v.11, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- Scientific Reports
- Volume
- 11
- Number
- 1
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/51700
- DOI
- 10.1038/s41598-020-80514-8
- ISSN
- 2045-2322
- Abstract
- Isolation of pure extracellular vesicles (EVs), especially from blood, has been a major challenge in the field of EV research. The presence of lipoproteins and soluble proteins often hinders the isolation of high purity EVs upon utilization of conventional separation methods. To circumvent such problems, we designed a single-step dual size-exclusion chromatography (dSEC) column for effective isolation of highly pure EVs from bone marrow derived human plasma. With an aim to select appropriate column design parameters, we analyzed the physiochemical properties of the major substances in bone marrow derived plasma, which include EVs, lipoproteins, and soluble proteins. Based on these findings, we devised a novel dSEC column with two different types of porous beads sequentially stacked each other for efficient separation of EVs from other contaminants. The newly developed dSEC columns exhibited better performance in isolating highly pure EVs from AML plasma in comparison to conventional isolation methods. © 2021, The Author(s).
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 2. Clinical Science > Department of Thoracic and Cardiovascular Surgery > 1. Journal Articles
- 4. Research institute > Korea Artificial Organ Center > 1. Journal Articles
- 2. Clinical Science > Department of Medical Oncology and Hematology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.