杜沖 (Eucommiae Cortex) 抽出物이 動物性 癌細胞 增殖抑制에 미치는 影響

Abstract

This study was devised to observe the cytotoxic activity of Eucommial cortex and leaf extracts against the leukemic cell, L_(1210), in vitro. Each extract was prepared by extraction with water, 50% and 95% ethyl alcohol, acetone, chloroform and petroleum ether, and then the cytotoxic activity of each extract was compared with control group and each other. The effects incubation time, size distribution of cell, cloning efficiency, and development of resistance in the culture medium against the growth of L_(1210) were determined after treatment of chloroform extract which had showed strongest activity. The experimental results obtained are summarized as follows; 1. The cytotoxic activity of Eucommial cortex and leaf was strongest in the chloroform extract and that of water was weakest. 2. The specific activity of chloroform extract of Eucommial leaf was stronger than that of Eucommial cortex extract. 3. The cytotoxic activity of Eucommial cortex showed the highest value within 12 hours during incubation, but the activity of Eucommial leaf extract increased gradually in course of incubation time. 4. When the L_(1210) cell were incubated with extracts of Eucommial cortex and leaf for 24 hours, the peak of size distribution of cell was shifted to direction of the small size region. 5. When L_(1210) cells were incubated in media containing 3 units or 1.5 units of extract of Eucommial cortex or leaf, viable cells estimated by the soft agar cloning method were not present. 6. The stability of the Eucommial leaf extract in the culture medium of L_(1210) cell was more stable than Eucommial cortex extract.