Studies on platelet aggregability in thrombotic disease and hypercholesterolemia and effects of aspirin and dipyridamole

Abstract

Although platelet have been implicated in the pathogenesis of the thrombotic disease, the platelet aggregability was not well studied in Korea. Author measured platelet aggregability in 103 clinical cases including 30 healthy volunteers to evaluate the platelet function and the effect of Aspirin and Dipyridamole on aggregability In Korean. 24 patients with cerebral thrombosis, 24 Patients with ischemic heart disease and 25 patients with hypercholesterolemia were included for this study. Aggregation tests were performed at three final concentrations of epinephrine (10uM/L) and ADP (4 uM/L, 10 uM/L) with platelet aggregometer which was made by Chrono-Log Corp. in all cases. Platelet aggregations were measured in patients who were treated with Aspirin, Dipyridamole and combined treatment of Aspirin and Dipyridamole respectively. The following results were obtained. 1 The mean maximal platelet aggregability in the normal subjects induced by 10 uM/L epinephrine was 59.3±24.25%, 66.6±14.00% in Bm and 62.5±19.30% in B5 in induction by 4 uM/L ADP, and 77.2±8.99% in Bm ad 76.6±9.83% in B5 in induction by 10 uM/L ADP. 2. The mean maximal platelet aggregability in patients with cerebral thrombosis induced by 10 uM/L epinephrine was 89.2±7.33%, 78.8±9.41% in Bm and 78.5±9.93% in B5 in induction by 4uM/L ADP, and 86.4±7.69% in Bm and B5 in induction by 10 uM/L ADP. The results showed significantly elevated platelet aggregability than that of normal subjects.(p<0.01) 3. The mean maximal platelet aggregability in patients with ischemic heart disease induced by 10 uM/L epinephrine was 88.1±11.99%, 78.2±12.50% in Bm and 76.3±15.66% in B5 in induction by 4 uM/L ADP, and 86.4±8.63% in Bm and B5 in induced by 10 uM/L ADP. The results showed significantly elevated aggregability than that of normal subjects.(p<0.01) 4. The mean maximal platelet aggregability in patients with hypercholesterolemia induced by 10 uM/L epinephrine was 86.8±15.99%, 82.7±11.19% in Bm and 82.0±12.87% in B5 in induction by 4 uM/LADP, and 88.5±11.47% in Bm and B5 in induction by 10 uM/L ADP. The results showed significantly elevated platelet aggregability than that of normal subject. (p<0.01) 5. The mean maximal platelet aggregability in patients with thrombotic disease was studied by Dipyridamole administration. The platelet aggregability induced by epinephrine before administration was 90.9±8.52% and after administration it was 78.9±15.68%, and the results showed that Dipyridamole lowered aggregability significantly. The platelet aggregability induced by 4 uM/L ADP before administration was 84.0±11.90% in Bm and B5 and after administration it was 78.0±11.44% in Bm and B5, and the results showed that Dipyridamole lowered aggregability but not significant. The platelet aggregability induced by 10 uM/L ADP before administration was 89.2±10.39% in Bm and B5 and after administration it was 80.5±8.44% in Bm and B5, and the results showed that Dipyridamole lowered aggregability significantly. 6. The mean maximal platelet aggregability in patients with thrombotic disease was studied by Aspirin administration. The platelet aggregability induced by epinephrine before administration was 91.0±4.79% and after administration it was 47.6±17.72%. The Platelet aggregability induced by 4 uM/L ADP before administration was 84.6± 10.37% in Bm and B5 and after administration it was 72.6± 11.85% in Bm and 65.3±15.97% in B5. The Platelet aggregability induced by 10 uM/L ADP before administration was 84.9±6.30% in Bm and B5 and after administration it was 77.7±8.60% in Bm and 75.0±8.89%. The results showed that Aspirin lowered aggregability markedly. 7. The mean maximal platelet aggregability in patients with thrombotic disease was studied by combined administration of Aspirin and Dipyridamole. The platelet aggregability induced by epinephrine before administration was 86.7±13.77% and after administration it was 36.7± 14.01%. The Platelet aggregability induced by 4 uM/L ADP before administration