Performance Evaluation of Alere i Influenza A&B in Detecting Influenza Viruses A and B
- Authors
- Kim, Ha Nui; Jang, Woong Sik; Nam, Jeong hun; Mihn, Do-Cic; Lim, Chae Seung
- Issue Date
- Jan-2021
- Publisher
- NLM (Medline)
- Keywords
- Alere i Influenza A&B; influenza; Real-time PCR; RIDT
- Citation
- Annals of clinical and laboratory science, v.51, no.1, pp 106 - 111
- Pages
- 6
- Indexed
- SCIE
SCOPUS
- Journal Title
- Annals of clinical and laboratory science
- Volume
- 51
- Number
- 1
- Start Page
- 106
- End Page
- 111
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/52932
- ISSN
- 0091-7370
1550-8080
- Abstract
- OBJECTIVE: Alere i Influenza A&B is an isothermal nucleic acid amplification-based integrated system used for detecting and differentiating between influenza virus A and influenza virus B. We evaluated the clinical performances of Alere i Influenza A&B compared to that of real-time PCR, multiplex real-time PCR, and two rapid influenza diagnostic kits. METHODS: Nasopharyngeal aspiration specimens (n=315) from patients with signs of acute respiratory infection were collected between 2015 and 2016. Samples were tested using real-time PCR, the multiplex RT-PCR Anyplex II RV16 Detection kit, Alere i Influenza A&B, BD Veritor™ System Flu A+B, and the Sofia Influenza A+B Fluorescence Immunoassay. Positive influenza specimens detected by the Anyplex II RV16 Detection kit were tested by real-time PCR. RESULTS: Compared to that of multiplex RT-PCR (influenza A, n=88; influenza B, n=82; influenza-negative, n=145), the sensitivities of Alere i, Sofia, and Veritor for influenza A were 97.7%, 72.7%, and 71.6%, respectively, whereas for influenza B, the sensitivities were 96.3%, 80.4%, and 75.6%, respectively. The specificity of Alere i, Sofia, and Veritor was 100.0%. CONCLUSIONS: The clinical performance of Alere i Influenza A&B is satisfactory, with the advantage of a significantly shorter test time than other molecular assays. It is suitable for point-of-care testing and rapid influenza diagnostic tests because of its high sensitivity and specificity. Copyright © 2021 by the Association of Clinical Scientists, Inc.
- Files in This Item
-
Go to Link
- Appears in
Collections - 4. Research institute > Institute for Trauma Research > 1. Journal Articles
- 2. Clinical Science > Department of Laboratory Medicine > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.