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Two-Photon Probes for Lysosomes and Mitochondria: Simultaneous Detection of Lysosomes and Mitochondria in Live Tissues by Dual-Color Two-Photon Microscopy Imaging
- Issue Date
- Oct-2015
- Publisher
- John Wiley and Sons Ltd
- Keywords
- fluorescent probes; imaging agents; lysosomes; mitochondria; two photon
- Citation
- Chemistry - An Asian Journal, v.10, no.10, pp 2240 - 2249
- Pages
- 10
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Chemistry - An Asian Journal
- Volume
- 10
- Number
- 10
- Start Page
- 2240
- End Page
- 2249
- ISSN
- 1861-4728
1861-471X
- Abstract
- Novel two-photon (TP) probes were developed for lysosomes (PLT-yellow) and mitochondria (BMT-blue and PMT-yellow). These probes emitted strong TP-excited fluorescence in cells at widely separated wavelength regions and displayed high organelle selectivity, good cell permeability, low cytotoxicity, and pH insensitivity. The BMT-blue and PLT-yellow probes could be utilized to detect lysosomes and mitochondria simultaneously in live tissues by using dual-color two-photon microscopy, with minimum interference from each other. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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