Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression
- Authors
- Lee, Sehe Dong; Choe, Jung Wan; Lee, Beom Jae; Kang, Myoung Hee; Joo, Moon Kyung; Kim, Ji Hoon; Yeon, Jong Eun; Park, Jong Jae; Kim, Jae Seon; Bak, Young Tae
- Issue Date
- Jan-2015
- Publisher
- Baishideng Publishing Group
- Keywords
- Butein; Colitis; Inflammatory bowel disease; Interleukin-6/signal transducer and activator of transcription 3; Matrix metalloproteinase-9
- Citation
- World Journal of Gastroenterology, v.21, no.2, pp 465 - 474
- Pages
- 10
- Indexed
- SCIE
SCOPUS
- Journal Title
- World Journal of Gastroenterology
- Volume
- 21
- Number
- 2
- Start Page
- 465
- End Page
- 474
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/54134
- DOI
- 10.3748/wjg.v21.i2.465
- ISSN
- 1007-9327
2219-2840
- Abstract
- AIM: To evaluate the effects of butein on inflammatory cytokines, matrix metalloproteinase-9 (MMP-9), and colitis in interleukin (IL)-10(-/-) mice.
METHODS: To synchronize colitis, 8- to 10-wk-old IL-10(-/-) mice were fed pellet-chow containing piroxicam for 2 wk. Subsequently, phosphate-buffered saline or butein (1 mg/kg per day, ip) was injected for 4 wk. Histologic scores, inflammatory cytokines, MMP-9 and phosphorylated signal transducer and activator of transcription 3 (pSTAT3) expressions were analyzed in IL-10(-/-) mice and in Colo 205 cells.
RESULTS: Butein reduced the colonic inflammatory score by > 50%. Expression levels of IL-6, IL-1 beta, interferon (IFN)-gamma and MMP-9 were decreased in the colons of mice exposed to butein, whereas other inflammatory cytokines (IL-17A, IL-21 and IL-22) were unchanged. Immunohistochemical staining for pSTAT3 and MMP-9 was significantly decreased in the butein-treated groups compared with the controls. Butein inhibited IL-6-induced activation of STAT3 in Colo 205 cells.
CONCLUSION: Butein ameliorated colitis in IL-10(-/-) mice by regulating IL-6/STAT3 and MMP-9 activation.
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- Appears in
Collections - 2. Clinical Science > Department of Internal Medicine > 1. Journal Articles
- 2. Clinical Science > Department of Gastroenterology and Hepatology > 1. Journal Articles
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